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Plasmid#167515PurposeBroad-Host range expression vector, RK2 and pUC origin, CymRAM repressor, PCymRC promoter controlling GmR, Kanamycin Resistance Marker.DepositorInsertCymRAM-GmR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLO43.3.-6_human ProCas9Flavi-6
Plasmid#121630PurposeU6-sgRNAdest_CMV-intron_hProCas9Flavi-6_T2A Mcherry_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKCyR6
Plasmid#149493PurposeBroad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.DepositorInsertCymR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCymRCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTK421
Plasmid#114688PurposeRFP-Nano tagging at NuMA's C-terminal locusDepositorInsertRFP-Nano
UseTagsExpressionMutationPromoterAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI
Plasmid#48975Purposefor Cre-lox cassette exchange of untagged gene sequences into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIVR5
Plasmid#167510PurposeBroad-Host range expression vector, pUC origin, Tn7 recognition sites, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertVanRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPVanCCAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLO43.3_human ProCas9Flavi
Plasmid#121629PurposeU6-sgRNAdest_CMV-intron_hProCas9Flavi_T2A Mcherry_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCM54
Plasmid#220830PurposeEryA sensor with increased sensitivity due to phosphorylation of EryA by mphA, which traps EryA in cellDepositorInsertmphA
UseTagsExpressionMutationPromoterPmphAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM98
Plasmid#220831PurposeEryA sensor with further increased sensitivity by mutation of mphR (T17R), in addition to mphA phosphorylationDepositorInsertmphR
UseTagsExpressionMutationT17RPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKPcR5
Plasmid#149467PurposeBroad-Host range expression vector, RK2 and pUC origin, PcaU(AM) repressor, P3B5B promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertPcaU(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP3B5BAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKVR5
Plasmid#149469PurposeBroad-Host range expression vector, RK2 and pUC origin, VanR(AM) repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertVanR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPVanCCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJKR-H-tetR
Plasmid#62561PurposeTetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED17x11
Plasmid#134814PurposesfGFP expression plasmid for characterizing qtRNA chargingDepositorInsertsfGFP-151-TAGA
UseSynthetic BiologyTags6xHisExpressionBacterialMutation151-TAGAPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM5411
Plasmid#132663PurposeRSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
UseTagsExpressionBacterialMutationPromoterPaadA and PconIIAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b61
Plasmid#58310PurposeShuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
KG#908
Plasmid#110914PurposeExpresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegansDepositorInsertsrab-3 promoter
TIR1 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-mphR
Plasmid#62559PurposeMacrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-araC
Plasmid#62563PurposeArabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7sgRNA
Plasmid#111820PurposeExpresses sgRNA in T. brucei cellsDepositorInsertsgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only