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pSLIK-rABE-Flag

Plasmid

#191383

Purpose

Doxycycline inducible expression of RNA A-to-I editor rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

rABE-Flag

Use

Lentiviral and Synthetic Biology

Tags

Flag

Expression

Mammalian

Promoter

tet-inducible promoter

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pChREBP

Plasmid

#39235

Depositor

Isabelle Leclerc

Article

da Silva Xavier et al J Lipid Res. 2006 Nov;47(11):2482-91. Ep

Insert

ChREBP (Mlxipl Mouse)

Tags

cMyc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
pJK1

Plasmid

#111722

Purpose

to express human BLM protein in yeast cells

Depositor

Ian Hickson

Article

Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

Insert

BLM (BLM Human)

Tags

hexahistidine

Expression

Yeast

Mutation

a C-terminal hexahistidine tag was added, a yeast…

Promoter

GAL1

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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HC-M

Plasmid

#173481

Purpose

An rrnB P1-based GFP ATP reporter in E. coli

Depositor

Rahul Sarpeshkar

Article

Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

Insert

GFP-mut2

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

rrnB P1

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-WT-STAT1

Plasmid

#71454

Purpose

Lentiviral expression of WT-STAT1

Depositor

George Stark

Article

Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

Insert

STAT1 (STAT1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-GCaMP6m-Xn

Plasmid

#118976

Purpose

Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus

Depositor

Xiaodong Liu

Article

Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

Insert

GCaMP6m-XN

Expression

Mammalian

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO eGFP Puro (w159-1)

Plasmid

#17481

Purpose

3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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ITSN-mcherry-sspB

Plasmid

#85220

Purpose

Role of Cdc-42 selective GEF domain from ITSN in immune cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

ITSN1(1225-1575) (ITSN1 Human)
mcherry
sspB

Tags

NA

Expression

Mammalian

Mutation

R73Q

Promoter

CMV

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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BC1177-pHR-SFFV-GFP(1-10)-NLS

Plasmid

#164039

Purpose

Expression of GFP1-10 in mammalian cells

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

GFP(1-10)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEGFP-Kan

Plasmid

#44900

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pK170.AAV-TRE-Cre-WPRE (Supernova)

Plasmid

#85040

Purpose

For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc. Inducible with tetracycline or doxycycline.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nlsCre-WPRE

Use

AAV

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pRPN265NB-LaG16-MNase

Plasmid

#170978

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

Insert

Glutathione-S-Transferase/LaG16/MNase

Tags

GST

Expression

Bacterial

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIP-cathepsinD-YMH

Plasmid

#182644

Purpose

Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

Depositor

Martin Spiess

Article

Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

Insert

cathepsinD

Use

Retroviral

Tags

His6-tag, TS site from proCCK, and myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Golgi-Ras-LOCKR-S

Plasmid

#216499

Purpose

FRET-based reporter for Ras activity, Golgi localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Golgi-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/Strep-HA-ZAK beta

Plasmid

#141195

Purpose

Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 isoform 2 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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CROP-seq-mTurquoise2-v2-no-scaffold

Plasmid

#228522

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Insert

mTurquoise2

Use

Lentiviral

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX-307_LgBiT

Plasmid

#191211

Purpose

Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

LgBiT (large bit)

Use

Lentiviral and Luciferase

Mutation

AB030246.1 (mutated in PMID 26569370)

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

Plasmid

#209077

Purpose

Ngn2 mediated cortical neuron induction, dox induction is included

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
SNAPtag

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-TeNT

Plasmid

#240990

Purpose

Expresses mCherry-TeNT in mammalian cells; for AAV transduction of neurons

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

mCherry-V5-TeNT

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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