We narrowed to 16,183 results for: nes

Showing: 10981 - 11000 of 16183 results

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p70rg 11

Plasmid

#17830

Depositor

Jay Keasling

Publication

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
p_sc-eVIP-delta-puro-EGFP-tdTomato

Plasmid

#249437

Purpose

Transfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systems

Depositor

Nils Blüthgen

Publication

Hollek et al Mol Syst Biol. 2026 Jan 19. doi: 10.1038

Insert

tdTomato

Use

Lentiviral

Promoter

EF-1a

Available since

Sept. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_3x_U6_Cldn5_CAG_nlsTagBFP2

Plasmid

#247679

Purpose

Targeting Cldn5 gene in vivo by adeno-associated viral vector-mediated delivery

Depositor

Benoit Vanhollebeke

Publication

Panji et al Commun Biol. 2026 Feb 25;9(1):479. doi:

Insert

Cldn5 sgRNAs

Use

AAV, CRISPR, and Mouse Targeting

Tags

nls-TagBFP2

Expression

Mammalian

Promoter

U6

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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PLKO.1-SNORA13-H/ACA Mut

Plasmid

#255004

Purpose

Express SNORA13 with mutations at the H box and ACA box in mammalian cells

Depositor

Joshua Mendell

Publication

Cheng et al Cell. 2024 Aug 22;187(17):4770-4789.e23.

Insert

SNORA13-H/ACA Mut (SNORA13 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

H box and ACA box

Promoter

U6

Available since

May 15, 2026

Availability

Academic Institutions and Nonprofits only

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XLone-EPB41L4A-AS1 with SNORA13

Plasmid

#255000

Purpose

Express EPB41L4A-AS1 with SNORA13 located in the intron in mammalian cells

Depositor

Joshua Mendell

Publication

Cheng et al Cell. 2024 Aug 22;187(17):4770-4789.e23.

Insert

EPB41L4A-AS1 with SNORA13 (EPB41L4A-AS1 Human)

Expression

Mammalian

Promoter

TRE3G

Available since

May 15, 2026

Availability

Academic Institutions and Nonprofits only

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PLKO.1-SNORA13-H/ACA Del

Plasmid

#255005

Purpose

Express SNORA13 with deletions at the H box and ACA box in mammalian cells

Depositor

Joshua Mendell

Publication

Cheng et al Cell. 2024 Aug 22;187(17):4770-4789.e23.

Insert

SNORA13-H/ACA Del (SNORA13 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

H box and ACA box

Promoter

U6

Available since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Renilla-FLAG-firefly

Plasmid

#255006

Purpose

Used as a control for Plasmid pcDNA3 Renilla-FLAG-firefly-K529E

Depositor

Joshua Mendell

Publication

Cheng et al Cell. 2024 Aug 22;187(17):4770-4789.e23.

Insert

Renilla-FLAG-firefly

Use

Luciferase

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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ARL13B-(R200C)-GFP

Plasmid

#246841

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with R200C mutation

Depositor

Lei Lu

Publication

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Arginine 200 to Cysteine

Available since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-GFP(1-245)

Plasmid

#246847

Purpose

Express Arl13B (resistance to sgRNA), aa1-245, tagged with GFP

Depositor

Lei Lu

Publication

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 246-428

Available since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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iPE-D10A-Cas9

Plasmid

#239380

Purpose

For inverse prime editor using nCas9-D10A in HEK293T cells

Depositor

Caixia Gao

Publication

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

nCas9-D10A, M-MLV RT

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D10A in Cas9

Promoter

CMV

Available since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHS23_10xHis_MBP_TEV_SpG-Cas9

Plasmid

#244826

Purpose

Bacterial expression of SpG Cas9 for protein purification

Depositor

Jennifer Doudna

Publication

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-TO-Δ37-HA PPM1H

Plasmid

#208372

Purpose

Lentiviral expression of HA-tagged human Δ37-PPM1H

Depositor

Suzanne Pfeffer

Publication

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

delta37-HA PPM1H (PPM1H Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta37

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD_beta-gamma

Plasmid

#226981

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD_beta-gamma

Tags

His6 (on backbone)

Expression

Bacterial

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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CSII-PPM1H-mApple-mito

Plasmid

#207351

Purpose

Mammalian expression of PPm1H-mito

Depositor

Suzanne Pfeffer

Publication

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPm1H-mito (PPM1H Human)

Expression

Mammalian

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-PPM1H S124A

Plasmid

#208368

Purpose

Mammalian expression of HA-tagged human PPM1H S124A

Depositor

Suzanne Pfeffer

Publication

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-PPM1H S124A (PPM1H Human)

Expression

Mammalian

Mutation

S124A

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-PPM1H S211A

Plasmid

#208369

Purpose

Mammalian expression of HA-tagged human PPM1H S211A

Depositor

Suzanne Pfeffer

Publication

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-PPM1H S211A (PPM1H Human)

Expression

Mammalian

Mutation

S211A

Available since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HeFSpCas9-NLS-6xHis

Plasmid

#207391

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HeFSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

T7

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9_nonPuro_OSS

Plasmid

#186652

Purpose

Cas9 plasmid to express the Cas9 in OSS cells

Depositor

Astrid Haase

Publication

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Cas9 Expression Plasmid

Expression

Insect

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRD400

Plasmid

#163107

Purpose

Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Publication

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_eYFP_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD188

Plasmid

#163099

Purpose

Expression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Publication

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mEos3.2_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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