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ATG9A-EGFP

Plasmid

#210367

Purpose

Expresses ATG9A fused with EGFP in mammalian cells

Depositor

Michele Pagano

Publication

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATG9A (ATG9A Human)

Tags

EGFP

Expression

Mammalian

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-4 dCARD

Plasmid

#208785

Purpose

Inducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2

Depositor

Petr Broz

Publication

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cry2 mCherry Caspase4 dCARD (CASP4 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

CARD domain is removed

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-HMGB1-WT

Plasmid

#194561

Purpose

Dox-inducible Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion Protein

Depositor

Denes Hnisz

Publication

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)

Plasmid

#174526

Purpose

Dual-fluorescence reporter for quantifying PylT(UAG) decoding efficiency

Depositor

Yu-Hsuan Tsai

Publication

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PylS

Tags

Flag

Expression

Mammalian

Promoter

EF-1α

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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2xmyc-JIP4

Plasmid

#164633

Purpose

Expresses JIP4 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Publication

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Tags

2xmyc

Expression

Mammalian

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI-CMV-GFP-TREX1 D18N BLAST

Plasmid

#164225

Purpose

lentiviral plasmid for GFP-hTREX1 D18N (catalytically-deficient)

Depositor

John Maciejowski

Publication

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D18N

Promoter

CMV

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pEXPqcxip-hFTSJ3-FLAG

Plasmid

#165463

Purpose

Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.

Depositor

Martin Dorf

Publication

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)

Use

Retroviral; Gateway

Tags

FLAG

Expression

Mammalian

Promoter

CMV/MSV, 5'LTR

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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FH1-MGT#2-mCherry_PGK-H2B-CFP

Plasmid

#164102

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Publication

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Plasmid

#159849

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag

Plasmid

#132402

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-BE4-PpAPOBEC1 H122A

Plasmid

#138338

Purpose

Mammalian expression plasmid for BE4-PpABOBEC1H122A

Depositor

Nicole Gaudelli

Publication

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BE4-PpAPOBEC1 H122A

Use

Synthetic Biology

Tags

BP-NLS

Mutation

Change of His122 to Ala.

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag

Plasmid

#132401

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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LADL Anchor

Plasmid

#127664

Purpose

Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Publication

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-CIBN-2A-Puro

Tags

3XFLAG

Expression

Mammalian

Mutation

Cas9 (D10A,H840A) mutant

Promoter

EF1a

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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hU6 promoter PspCas13b gRNA dual luciferase reporter 3’UTR

Plasmid

#119859

Purpose

gRNA plasmid with hU6 promoter PspCas13b gRNA dual luciferase reporter

Depositor

Bryan Dickinson

Publication

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA dual luciferase reporter

Use

CRISPR

Expression

Mammalian

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-mCherry HDRT Source (pTR 144)

Plasmid

#112013

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

Depositor

Alexander Marson

Publication

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAB11A-mCherry HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Non-specific sgRNA

Plasmid

#109432

Purpose

MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

Depositor

Reuben Harris

Publication

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Non-specific gRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_AIFM1

Plasmid

#111659

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

AIFM1 (AIFM1 Human)

Expression

Mammalian

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-Myc-CRBN-pGK-HYG

Plasmid

#107376

Purpose

Lentiviral expression of myc-CRBN

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

UbcP

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Publication

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p7135 pHAGE-P-CMVt-N-HA-GAW-EIF3E

Plasmid

#100147

Purpose

Expresses EIF3E

Depositor

Peter Howley

Publication

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

EIF3E (EIF3E Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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