173,296 results
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Plasmid#207791PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.DepositorInsertsgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1
Plasmid#20668PurposeS. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.DepositorTypeEmpty backboneTagsGFP mut3ExpressionBacterialAvailable SinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-Flag-Smad3
Plasmid#11742DepositorAvailable SinceAug. 7, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pP2A-mCherry-N1
Plasmid#84329PurposeEncodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherryDepositorInsertP2A-mCherry
ExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-ADRA2A
Plasmid#190753PurposeExpression of ADRA2A with mEGFP tag on N-terminus.DepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPObooster
Plasmid#216160PurposePlasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strainsDepositorExpressionYeastMutationMKT1 gene containing an aspartic acid to glycine …Promoterendogenous promoterAvailable SinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR87-Tango
Plasmid#66379PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-Raichu-454HRasCT
Plasmid#176029PurposeA FRET biosensor for HRas activityDepositorInsertRaichu-Ras-HRasCT
ExpressionMammalianAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA-sfGFP
Plasmid#162689PurposepFA vector control expressing sfGFPDepositorInsertsfGFP
TagsC terminal 6x His tagExpressionBacterialPromoterPL tet promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-NLS-R-GECO
Plasmid#32462PurposeMammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorInsertR-GECO1.0
TagsNLS sequence DPKKKRKVExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-EGFP
Plasmid#71666PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
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YY206: pAAV_hSyn::GEMINI(B)-p2A-GEMINI(A)_UbC::HaloTag-GEMINI(A)
Plasmid#228886PurposeAAV plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a hSyn promoter, and HaloTag-GEMINI(A) under the control of a UbC promoter.DepositorInsertGEMINI(A); GEMINI(B); HaloTag-GEMINI(A)
UseAAVTagsHaloTagExpressionMammalianPromoterhSyn; UbcAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPIC9K_A2AR_316_V229C_R291A
Plasmid#246917PurposeExpress A2AR mutants in Pichia PastorisDepositorInsertAdenosine A2A receptor (ADORA2A Human)
Tags10xHis and alpha-factor secretion signal, FLAGExpressionYeastMutationtruncated to aa 1-316, V229C, R291APromoterAOX1Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI-EGFP-NR2b wt
Plasmid#45447DepositorInsertNR2B (Grin2b Rat)
TagsEGFPExpressionMammalianMutationEGFP inserted after the predicted signal peptide …PromoterCMVAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFPC1-huNFATc1EE-WT
Plasmid#24219DepositorAvailable SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR' CMV GFP
Plasmid#14858DepositorInsertCMV-GFP
UseLentiviralExpressionMammalianAvailable SinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
pALPS
Plasmid#90996PurposeLentiviral vector for expression of heterologous genesDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterSFFVAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-P2A-CIAR_pLenti_CMV_Puro
Plasmid#86503PurposeLenti plasmid for generating CIAR expressing stable cell linesDepositorInsertGFP-P2A-CIAR (SOS1 Human)
UseLentiviralExpressionMammalianMutationT968L in SOScatPromoterCMVAvailable SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-LaminB_C1
Plasmid#189776PurposeConstruct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusionDepositorInsertmScarlet3-LaminB
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only