159,530 results

1. pKanCMV-mClover3-mRuby3

   Plasmid

   #74252

   Purpose

   Mammalian expression of tandem fusion of mClover3-mRuby3

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   mClover3-mRuby3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV3Tag8li-hTXLNA-3xFLAG

   Plasmid

   #165471

   Purpose

   Human TXLNA mammalian expression vector with 3 C-terminal FLAG tags.

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   taxilin alpha (TXLNA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFPN1-human Dynamin 1aa

   Plasmid

   #22163

   Depositor

   Pietro De Camilli

   Article

   Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

   Insert

   human Dynamin 1aa (DNM1 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   D744N

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDECKO_GFP

   Plasmid

   #72619

   Purpose

   Expresses two gRNAs targeting GFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a-EGFP (no signal control)

   Plasmid

   #170167

   Purpose

   Mammalian Expression of no signal control

   Depositor

   Christopher Moxham

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLR5-CBh-dCas9-hUtx-IRES-Hyg

   Plasmid

   #122374

   Purpose

   Expresses dCas9-hUtx-IRES-Hyg in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   CBh-dCas9-hUtx-IRES-Hyg-pA
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOlyA(WT)

   Plasmid

   #132391

   Purpose

   Ostreolysin A for sensing sphingomyelin/cholesterol complexes

   Depositor

   Arun Radhakrishnan

   Article

   Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

   Insert

   Ostreolysin A wild-type
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: C62S, C94S, S151C

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB700

   Plasmid

   #64046

   Purpose

   Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CAGGS

   Available since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Calnexin-N-14

   Plasmid

   #55005

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   I474N in Calnexin

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. LCV2 IRF3 KO

    Plasmid

    #217446

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    sgRNA targeting IRF3 (IRF3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MC4R-Tango

    Plasmid

    #66430

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MC4R (MC4R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV5B-Flag-Smurf2 wt

    Plasmid

    #11746

    Depositor

    Jeff Wrana

    Article

    Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

    Insert

    SMURF2 (SMURF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. 2C-3XtbGFP-PEST

    Plasmid

    #69072

    Purpose

    Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    turboGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

    Plasmid

    #229828

    Purpose

    A Piggybac compatible, cre dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE

    Plasmid

    #101852

    Purpose

    Direct reprogramming of adult fibroblasts to neurons

    Depositor

    Malin Parmar

    Article

    Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

    Insert

    Ascl1, Brn2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6, PGK

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCytochrome C-GFP

    Plasmid

    #41182

    Depositor

    Douglas Green

    Article

    Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

    Insert

    Cytochrome C
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBbA5a-sufABCDSE

    Plasmid

    #195056

    Purpose

    Expresses SUF operon from E. coli from IPTG-inducible promoter

    Depositor

    Greg Bokinsky

    Article

    D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

    Insert

    sufABCDSE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMHE-vhhGFP4-hIgG1-Fc

    Plasmid

    #105576

    Purpose

    T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG_smFP Myc

    Plasmid

    #59757

    Purpose

    encodes multiple copies of an epitope tag Myc

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _Myc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEMT-PTE2A

    Plasmid

    #111773

    Purpose

    Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Type

    Empty backbone

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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