We narrowed to 13,482 results for: cDNA

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eGFP-RAPH1-F2

Plasmid

#194861

Purpose

Express the RAPH1 fragment (535-868) tagged to eGFP in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acids 535-868

Promoter

CMV

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat-PPFPPFtoAAAAAA_AH

Plasmid

#148908

Purpose

Insect Expression of DmHPat-PPFPPFtoAAAAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmHPat-PPFPPFtoAAAAAA (Patr-1 Fly)

Expression

Insect

Mutation

three silent mutations compared to the sequence g…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBruno_N

Plasmid

#147028

Purpose

Insect Expression of DmBruno

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmBruno (aret Fly)

Expression

Insect

Mutation

three non silent mutations S88T, H203R and A449R …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBruno_N

Plasmid

#147030

Purpose

Insect Expression of DmBruno

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmBruno (aret Fly)

Expression

Insect

Mutation

three non silent mutations S88T, H203R and A449R …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat_57-968-dsRNAres_J

Plasmid

#146690

Purpose

Insect Expression of DmHPat_57-968-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation A1758G and one non silent G56…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat-del57-499-dsRNAres_J

Plasmid

#146691

Purpose

Insect Expression of DmHPat-del57-499-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat-del57-499-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation A1758G compared to the sequen…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat_57-968-del500-684_J

Plasmid

#146695

Purpose

Insect Expression of DmHPat_57-968-del500-684

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-del500-684 (Patr-1 Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmHPat_57-968-dsRNAres_H

Plasmid

#146464

Purpose

Insect Expression of DmHPat_57-968-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation T477C and one non silent muta…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pClathrin-miRFP718nano

Plasmid

#195747

Purpose

Clathrin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-clathrin light chain

Tags

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP718nano

Plasmid

#195746

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-human alpha-tubulin

Tags

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

Plasmid

#194725

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Mutation

D908A

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-CBS (H65R)

Plasmid

#182924

Purpose

Mammalian expression plasmid for human CBS mutant H65R

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

Cystathionine Beta-Synthase (CBS Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed histidine 65 to arginine

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-mVenusC-14-HP1b Chromo W42A

Plasmid

#191399

Purpose

W42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

FLAG-mVenusC-14-HP1b Chromo W42A

Tags

3xFLAG

Expression

Mammalian

Mutation

W42A

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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nPPARg429-gs10-NLS-VPR

Plasmid

#188266

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nPPARg429 (PPARG Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

Plasmid

#169592

Purpose

Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

phloretin-controlled SEAP and iRFP expression

Expression

Mammalian

Promoter

TtgO2-PCMVmin-2

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

Plasmid

#169589

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin-2

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2393-Tier1-PhCMV-p2a_fastFT

Plasmid

#169530

Purpose

Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused fluorescent timer, fast maturation time

Expression

Mammalian

Promoter

PhCMV

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTL370

Plasmid

#69880

Purpose

attB flanked genomic Cirl for integration at attP sites.

Depositor

Robert Kittel, Tobias Langenhan

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb21

Plasmid

#184679

Purpose

NIR-Fb to spike-Cov-19 RBD

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb21 (S Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pVH63-Tier1-PMLP-Citrine_p2a_SEAP

Plasmid

#169557

Purpose

Tier-1 vector encoding PMLP-driven Citrine-p2A-SEAP expression (PMLP-Citrine-p2A-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PMLP-driven citrine-P2A-SEAP fusion construct

Expression

Mammalian

Promoter

PMLP

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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