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pHH0103_FCHSD1-2/2

Plasmid

#91258

Purpose

Protein expression and purification of human SH3 domain construct FCHSD1-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FCHSD1-2/2 (FCHSD1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SORBS2-2/3

Plasmid

#91264

Purpose

Protein expression and purification of human SH3 domain construct SORBS2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS2-2/3 (SORBS2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCML 220

Plasmid

#98588

Purpose

pCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Type

Empty backbone

Expression

Bacterial

Promoter

pLacO

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Cka2

Plasmid

#99980

Purpose

Shuttle yeast Cka2 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Cka2 (CKA2 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Utp16

Plasmid

#99985

Purpose

Shuttle yeast Utp16 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp16 (BUD21 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHL

Plasmid

#64171

Purpose

harbours marker genes HIS3 and LEU2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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YCp33-YDL183c-HA

Plasmid

#70164

Purpose

yeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal end

Depositor

Karin Nowikovsky

Article

Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

Insert

YDL183c

Tags

3xHA

Expression

Yeast

Promoter

YDL183c (217 nucleotides upstream of the ATG)

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only
pDGB1_omega2R

Plasmid

#68237

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_R4cons_Y129H

Plasmid

#64302

Purpose

Conservative version of OmpA Redesign 4 with Y129H mutation

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons_Y129H

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

25 mutations to lipid-facing surface

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pHYBAD177

Plasmid

#61826

Purpose

TvoVMAC7 intein

Depositor

Hideo Iwai

Article

Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

Insert

TvoVMAC16 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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RCAS(A) cScx EnR (CT#745)

Plasmid

#13890

Depositor

Cliff Tabin

Article

Brent et al Cell. 2003 Apr 18. 113(2):235-48.

Insert

Scleraxis (SCX Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
RCAS(A) cScx VP16 (CT#747)

Plasmid

#13891

Depositor

Cliff Tabin

Article

Brent et al Cell. 2003 Apr 18. 113(2):235-48.

Insert

Scleraxis (SCX Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
Expression_vector RO1_1

Plasmid

#158010

Purpose

Expression vector for the de novo designed protein Expression_vector_RO1_1

Depositor

Tanja Kortemme

Article

Pan et al Science. 2020 Aug 28;369(6507):1132-1136

Insert

de novo designed protein RO1_1

Tags

6x His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pKMDDV_Plasmid_2b

Plasmid

#249057

Purpose

Plasmid 2b containing RFP with J306 scRNA binding site, GFP with J108 scRNA binding site and a constitutively expressed scRNA_GFP

Depositor

Domitilla Del Vecchio

Article

Aravind et al Cell Systems, 2026; 17

Inserts

scRNA
Fast Folding Green Fluorescent Protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117 and ptrc

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-BiP-mtagBFP2-KDEL

Plasmid

#253969

Purpose

Mammalian expression of ER marker

Depositor

Yusuke Hirabayashi

Article

Nakamura et al Nat Commun. 2025 Apr 17;16(1):3401. doi:

Insert

mTagBFP2

Tags

KDEL

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCDB24-XhoI_D40

Plasmid

#254737

Purpose

Design 40

Depositor

Brian Kuhlman

Article

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

Design 40

Tags

HIS and SUMO

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDB24-XhoI_NF

Plasmid

#254741

Purpose

Naive Fusion

Depositor

Brian Kuhlman

Article

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

Naive Fusion

Tags

HIS and SUMO

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDB24-XhoI_D42

Plasmid

#254738

Purpose

Design 42

Depositor

Brian Kuhlman

Article

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

Design 42

Tags

HIS and SUMO

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDB24-XhoI_D12

Plasmid

#252874

Purpose

Design 12

Depositor

Brian Kuhlman

Article

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

Design 12

Tags

HIS and SUMO

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCDB24-XhoI_D48

Plasmid

#254739

Purpose

Design 48

Depositor

Brian Kuhlman

Article

Shelby et al Bioconjug Chem. 2026 Mar 17. doi: 10.102

Insert

Design 48

Tags

HIS and SUMO

Expression

Bacterial

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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