We narrowed to 24,425 results for: lars;

1. EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)

   Plasmid

   #191175

   Purpose

   Mammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   CMV-TO:mCherry:s70587:EGFP (frameshifted)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP is frameshifted

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST(frt)(ATG_V5-APEX2_noSTOP)

   Plasmid

   #168207

   Purpose

   Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection

   Depositor

   Markus Hafner

   Article

   Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

   Type

   Empty backbone

   Use

   Unspecified; Pdest gateway

   Tags

   V5 APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFP.R375

   Plasmid

   #138245

   Purpose

   Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJUMP18-pheS_NOC

   Plasmid

   #127031

   Purpose

   Basic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Insert

   Part PheS_NOC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   pheS A294G promiscuous mutation

   Promoter

   Available since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. sm-9xGCN4-Renilla-MS2

   Plasmid

   #178009

   Purpose

   Expresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Gomez-Puerta et al Elife. 2022 Jun 22;11. pii: 75580. doi:

   Insert

   sm-9xGCN4
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOS030E: Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

   Plasmid

   #163071

   Purpose

   Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   ratiometric pHluorin
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOS019E: Entry vector for: ClopHensorN chloride sensor (cytosolic)

   Plasmid

   #163066

   Purpose

   Entry vector for: ClopHensorN chloride sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   ClopHensorN
   

   Use

   Gateway entry vector

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJUMP27ts[mCherry]-1A(lacZ)

   Plasmid

   #126980

   Purpose

   Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   RepA A56V thermosensitive mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBB-BT-Ubiquitin

   Plasmid

   #36098

   Depositor

   Ezra Burstein

   Article

   Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

   Insert

   Ubiquitin (RPS27A Human)
   

   Use

   Tags

   TB-tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA0.002

    Plasmid

    #119560

    Purpose

    Level 0 acceptor vector for promoters with lacZ compatible with blue/white screening, low copy number, pSC101 ori.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTK1_024

    Plasmid

    #123686

    Purpose

    Encodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector L2-C3 insulator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGB3_omega2

    Plasmid

    #68239

    Purpose

    GoldenBraid level 1 Omega plant expression vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/FRT-ChemoB-NAD

    Plasmid

    #193824

    Purpose

    CMV overexpression of ChemoB-NAD in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoB-NAD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EBFP2:N39Y, A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p7128 pHAGE-P-CMVt-N-HA-GAW-HLA-A

    Plasmid

    #100154

    Purpose

    Expresses HLA-A

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    HLA-A (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFH51

    Plasmid

    #128213

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. SERA1-bio-His

    Plasmid

    #50812

    Purpose

    Expresses enzymatically monobiotinylated full length SERA1

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    SERA1
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-NES-ZapCmR1.1

    Plasmid

    #59014

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCS2 Flag Smad3 T179V

    Plasmid

    #26997

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    T179V

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-FLAG-Fos AA

    Plasmid

    #8967

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S362A, S374A

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

20. MTK1_023

    Plasmid

    #123685

    Purpose

    Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector L1- B1 insulator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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