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We narrowed to 22,456 results for: his

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Showing: 11041 - 11060 of 22456 results

  5. pSBL_DK202

    Plasmid
    #156426
    Purpose
    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Tags
    EGFP-His
    Expression
    Mammalian
    Mutation
    aa 42-275
    Promoter
    CMV
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156426-plasmid-map-sequence-id-306303

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  8. TBXTA-c006

    Plasmid
    #139755
    Purpose
    For protein expression/crystallography (PDB:6F59)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-S224
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139755-plasmid-map-sequence-id-296509

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  17. pFP.R275

    Plasmid
    #138224
    Purpose
    Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SGK).PhoRadA.(TQL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138224-plasmid-map-sequence-id-273527

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  18. pFastBac HT JS-Munc18b

    Plasmid
    #135554
    Purpose
    Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.
    Depositor
    Jingshi Shen
    Article
    Yu et al Methods Mol Biol. 2019;1860:237-249. doi
    Insert
    Munc18b (Stxbp2 Rat)
    Tags
    6xHis tag and TEV site
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    March 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    135554-plasmid-map-sequence-id-272494

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  19. pET-FLAG-SpCas9-HF1-plus

    Plasmid
    #126775
    Purpose
    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126775-plasmid-map-sequence-id-437774

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Showing: 11041 - 11060 of 22456 results