We narrowed to 59,461 results for: %s

1. pHR-EF1Alpha-Tet-on 3G

   Plasmid

   #118592

   Purpose

   Expresses Tet-on 3G from the EF1Alpha promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Tet-on 3G
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

   Plasmid

   #174515

   Purpose

   Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Inserts

   Mm PylS
   MmtRNA-Pyl-opt(UGA)
   

   Expression

   Bacterial

   Mutation

   ASL from the serT gene (UGA anticodon) and recoded

   Promoter

   GlnS and lpp

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS0035

   Plasmid

   #131125

   Purpose

   Mammalian Target-AID expression vector

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   nCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Tags

   PmCDA1 and UGI

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV promoter

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

   Plasmid

   #225587

   Purpose

   Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-vhhGFP4-T2A-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF1a-donor_opt(R2Tg)-GFP(intron)

   Plasmid

   #223252

   Purpose

   Mammalian expression of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PEmaxRNaseHtrunc

   Plasmid

   #207858

   Purpose

   Mammalian expression of PEmaxDRNaseH prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PEmaxDRNaseH
   

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   M-MLVRTDRNAseHrt

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CMV-MFSD6-mNeon-Puro

   Plasmid

   #239955

   Purpose

   Lentiviral vector to generate mNeon-tagged MFSD6 stable expressing cell line under CMV promoter

   Depositor

   Jan Carette

   Article

   Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

   Insert

   MFSD6-mNeon
   

   Use

   Lentiviral

   Tags

   mNeon

   Expression

   Mammalian

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-MFSD6-Puro

   Plasmid

   #239953

   Purpose

   Lentiviral vector to generate MFSD6 stable expressing cell line under CMV promoter

   Depositor

   Jan Carette

   Article

   Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

   Insert

   MFSD6
   

   Use

   Lentiviral

   Tags

   myc-flag

   Expression

   Mammalian

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-MFSD8-Puro

   Plasmid

   #239954

   Purpose

   Lentiviral vector to generate MFSD8 stable expressing cell line under CMV promoter

   Depositor

   Jan Carette

   Article

   Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

   Insert

   MFSD8
   

   Use

   Lentiviral

   Tags

   myc-flag

   Expression

   Mammalian

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85006

    Purpose

    For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCCLc-MND-A0201-Mart1-SABR

    Plasmid

    #119052

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    A0201-Mart1-SABR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MND

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85008

    Purpose

    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-CAG-hCas3

    Plasmid

    #134920

    Purpose

    Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas3 with bpNLS
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEos3-dnRac1

    Plasmid

    #92040

    Purpose

    Expresses mEos3 fusion with dnRac1 (T17N).

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T17N (dominant negative)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEos3-caRac1

    Plasmid

    #92041

    Purpose

    Expresses mEos3 fusion with caRac1 (Q61L)

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L (constitutively active)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. VPX-mRNA

    Plasmid

    #227679

    Purpose

    plasmid for VPX mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    VPX (HIV2gp3 )
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pscAAV-Sox2-mStr

    Plasmid

    #135617

    Purpose

    Donor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    P2A-mStrawberry flanked by Sox2 homology arms (Sox2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    P2A-mStrawberry

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG_ATG101

    Plasmid

    #223796

    Purpose

    Expression of recombinant protein for purification (co-expression with ATG13)

    Depositor

    James Hurley, Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    ATG101 (ATG101 Human)
    

    Expression

    Mammalian

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. EXT1- D164A-pLJC2-3XFLAG

    Plasmid

    #215828

    Purpose

    Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    Exostosin glycosyltransferase 1 Mutant (EXT1 Human)
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mammalian

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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