We narrowed to 32,473 results for: fer
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pKL2-138
Plasmid#219413PurposeEstradiol-inducible expression of 42-10 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
ExpressionYeastMutationFour Arg to Ala mutations in zinc finger backboneAvailable SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-GST-EGFP-GPIBY55
Plasmid#213720PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.DepositorInsertEGFP
UseCRISPRTagsGSTPromoterCMVAvailable SinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-GST-EGFP-GPICEAM7
Plasmid#213721PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.DepositorInsertEGFP
UseCRISPRTagsGSTPromoterCMVAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
RC(14-3-3ε)-LgBit
Plasmid#208926PurposeRecognition domain for in vitro RSK-activity sensorDepositorAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-Halo-ER
Plasmid#214419PurposeER localization of low-affinity NFAST fused to Halo-ERDepositorInsertRspA-N
TagsHaloExpressionMammalianPromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TST-EGFP-GPIDAF
Plasmid#213708PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.DepositorInsertEGFP
TagsTwin-strep-tagExpressionMammalianPromoterhPGKAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TST-EGFP-GPIBY55
Plasmid#213707PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.DepositorInsertEGFP
TagsTwin-strep-tagExpressionMammalianPromoterhPGKAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-hXRCC1pd-HIS
Plasmid#206036PurposeBacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoterDepositorInserthXRCC1
TagsHISExpressionBacterialMutationS103A,R186A,S184A,S193A,S219A,S220A,S236A,S268APromoterT7Available SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR54
Plasmid#203330Purposeepisomal bioluminescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
ExpressionYeastAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB398
Plasmid#203634PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).DepositorInsertFB367+FB395
UseSynthetic BiologyAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-PHLPP2
Plasmid#203605PurposeReporter plasmid carrying PHLPP2 3'UTRDepositorInsertPH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)
UseLuciferaseExpressionMammalianPromoterSV40Available SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-27bT
Plasmid#203600PurposeReporter plasmid carrying miR-27b target sequenceDepositorInsertcomplementary sequence to miR27b-3p (MIR27B Human)
UseLuciferaseExpressionMammalianPromoterSV40Available SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWR24
Plasmid#202771Purposeepisomal bioluminescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coNAT
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
ExpressionYeastAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWR16
Plasmid#202711Purposeintegrating bioluminescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
URA3 and flanking sequence
ExpressionYeastAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB88
Plasmid#199321PurposeCombine with Gal4 to direct TeNL expressionDepositorInsert15xUAS::delta pes-10::::TeNL::let-858 3'UTR
ExpressionWormMutationKD for calcium is 250 nM, 1 synthetic intronPromoter15xUAS::delta pes-10Available SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB109
Plasmid#199322PurposeCombine with Gal4 to direct CaMBI 300 expressionDepositorInsert15xUAS::delta pes-10::::CaMBI::let-858 3'UTR
ExpressionWormMutationOrange CaMBI with 300 nM KD for calciumPromoter15xUAS:::delta pes-10Available SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-MD-mTurquoise
Plasmid#193094Purposeconstitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cellsDepositorInsertMD-mTurquoise
UseLentiviralExpressionMammalianPromoterUbcAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-C-TEV-HAT
Plasmid#202597PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianPromoterCAGAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOET3-C-TEV-Twin-Strep
Plasmid#203324PurposeGenerate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only
