We narrowed to 41,529 results for: TRO

1. pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

   Plasmid

   #159896

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-Hygro-rKIF5B-GFP

   Plasmid

   #210335

   Purpose

   Overexpression of rKIF5B

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   rKIF5B (Kif5b Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-6xHis-GFP(+24)

   Plasmid

   #199163

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+24)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB: PGK-H2B-mIFP-T2A-rTetR-GBP-polyA-zeo

   Plasmid

   #163084

   Purpose

   rTetR DNA binding domain fused to GBP (anti-GFP nanobody)

   Depositor

   Lacra Bintu

   Article

   Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

   Insert

   anti-GFP nanobody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSuper mouse Dicer3

   Plasmid

   #14778

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   Dicer shRNA (Dicer1 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

6. PB-EEA-g1-PGK-Puro

   Plasmid

   #102908

   Purpose

   PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-guide1-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV Puro-UBC:GFP

   Plasmid

   #68482

   Purpose

   Retrovirus expressing GFP driven by UBC promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR and UBC

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUW-mTurquoise

   Plasmid

   #193092

   Purpose

   constitutive expression of mTurquoise in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   mTurquoise
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. plentiCRISPR-sgGOT1

   Plasmid

   #72874

   Purpose

   The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   sgGOT1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pX330-sgRNA_Dgcr8_1

    Plasmid

    #73525

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

11. GFAP-Archon-EGFP

    Plasmid

    #187979

    Purpose

    Expresses the fluorescent voltage indicator Archon under the GFAP promoter

    Depositor

    Chris Dulla

    Article

    Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

    Insert

    Archon1-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA_186

    Plasmid

    #133458

    Purpose

    U6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFP

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FLAG-mTUT7

    Plasmid

    #60044

    Purpose

    Mammalian expression of FLAG-tagged mouse TUT7

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    TUT7 (Zcchc6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA-FRT-FAK-kinase30

    Plasmid

    #59124

    Purpose

    FAK fret control sensor without FERM domain with 30 nm linker

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR L1-vas2-Cre-L4

    Plasmid

    #62301

    Purpose

    Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Insert

    Cre recombinase under control of vasa promoter and terminator
    

    Use

    Tags

    HA and SV40 NLS

    Expression

    Insect

    Mutation

    Promoter

    vasa

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3.0_nucLAMA-F98 IRES EGFP

    Plasmid

    #136618

    Purpose

    Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-F98-NLS (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-L1PA8CHmneo

    Plasmid

    #69608

    Purpose

    Expresses a neomycin tagged codon optimized L1PA8

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

    Insert

    L1PA8
    

    Use

    Tags

    mneo retrotransposition cassette

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag Snail 2SA

    Plasmid

    #16219

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    2SA: S97A, S101A

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pRS416gT-GalL-Cas9

    Plasmid

    #79903

    Purpose

    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

    Depositor

    Ronald Davis

    Article

    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

    Inserts

    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPM1 promoter, GalL, and RPR1 promoter with TetO …

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAGM37443

    Plasmid

    #153218

    Purpose

    Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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