165,313 results

1. pX330-COSMC-KO

   Plasmid

   #80008

   Purpose

   gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   COSMC (C1GALT1C1 Human)
   

   Use

   CRISPR

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. phCMV-GALV-MTR

   Plasmid

   #163612

   Purpose

   Retro and Lentiviral transduction of primary human germinal center B cells

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Insert

   GaLV MTR envelope
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. tFucci(SA)5

   Plasmid

   #153520

   Purpose

   New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

   Insert

   tFucci(SA)5.
   

   Tags

   AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

   Expression

   Bacterial and Mammalian

   Promoter

   CAG

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

   Plasmid

   #153004

   Purpose

   Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (N) (G78)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. R777-E125 Hs.MAPK1

   Plasmid

   #70409

   Purpose

   Gateway ORF clone of human MAPK1 [NM_002745.4] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEMS-II-dCas13-APO1-YTH

   Plasmid

   #204767

   Purpose

   GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   dCas13-NLS-APO1-YTH-HA
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   Ef1alpha

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1-copGFP-T2A-Puro

   Plasmid

   #72263

   Purpose

   Express copGFP and puromycin resistance gene under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   copGFP-T2A-Puro
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   elongation factor-1

   Available Since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. ABE8e-NG

   Plasmid

   #226856

   Purpose

   Expresses ABE8e-NG base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   ABE8e-NG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. DNMT3A-dCas9

   Plasmid

   #100090

   Purpose

   DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   DNMT3A (DNMT3A Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-DNMT3A-dCas9-NLS

   Expression

   Mammalian

   Mutation

   includes aa 602-912

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIneo RYR-1

    Plasmid

    #75113

    Purpose

    Clone encoding rabbit ryanodine receptor in mammalian expression vector

    Depositor

    Paul Allen, Robert Dirksen

    Article

    Nakai et al J Biol Chem. 1998 May 29;273(22):13403-6

    Insert

    Ryanodine receptor 1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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