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RCAS(A) human p110 alpha E545K

Plasmid

#52211

Purpose

expresses human p110 alpha with E545K mutation in avian cells

Depositor

Peter Vogt

Article

Kang et al Proc Natl Acad Sci U S A. 2005 Jan 18;10

Insert

p110 alpha E545K (PIK3CA Human)

Use

Retroviral; Avian

Mutation

Glutamic Acid 545 to Lysine

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pVRc15_436

Plasmid

#49735

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS15_436

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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mTFP1-Rab4a-7

Plasmid

#55507

Purpose

Localization: Ras GTPase, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Rab4a (RAB4A Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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P6598 MSCV-IP N-HAonly TBPL1

Plasmid

#34896

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

TBPL1 (TBPL1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pAG176 SLC23A2 3'UTR mut

Plasmid

#12034

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N9 3'UTR mut (SLC23A2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pDOE11 mas:mTq2 transient

Plasmid

#124248

Purpose

Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected plant cells.

Depositor

Attila Fehér

Article

Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

Type

Empty backbone

Expression

Plant

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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RFN_EGFP_47

Plasmid

#60071

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only
pHH0103_PTK6-1/1

Plasmid

#91441

Purpose

Protein expression and purification of human SH3 domain construct PTK6-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PTK6-1/1 (PTK6 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/NSH3* (P651L) uGFP

Plasmid

#14562

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/NSH3* (P651L)

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
PB-BE3-3NLS

Plasmid

#119032

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Tags

3xFlag

Expression

Mammalian

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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px330-MRPsg4

Plasmid

#87192

Purpose

disruption of human RMRP gene via CRISPR-Cas9, guide 4

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

human MRP RNA CRISPR guide #4 (RMRP Human)

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJT23_GalL_BE-PAPAP

Plasmid

#143486

Purpose

Expresses BE-PAPAP in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-PAPAP

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAG181 NeuroD 3'UTR mut

Plasmid

#12039

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N14 3'UTR mut (NEUROD1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pSG5HA-MAGE-KA240,245

Plasmid

#89140

Purpose

mammalian expression of N-terminal HA-tagged full-length human MAGE-A11 amino acid residues 2-429 with KA240,KA245 ubiquitination sites mutated

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

MAGE-A11 (MAGEA11 Human)

Tags

HA

Expression

Mammalian

Mutation

K240A, K245A

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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TOPO pGK

Plasmid

#68452

Purpose

TOPO construct expressing pGK for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Phosphoglycerate Kinase 1 Promoter (PGK1 Human)

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-1/4

Plasmid

#91544

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-1/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-1/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-2/4

Plasmid

#91457

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-2/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-2/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-4/4

Plasmid

#91374

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-4/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-4/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF3-2/2

Plasmid

#91356

Purpose

Protein expression and purification of human SH3 domain construct SH3RF3-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF3-2/2 (SH3RF3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3RF1-3/4

Plasmid

#91366

Purpose

Protein expression and purification of human SH3 domain construct SH3RF1-3/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3RF1-3/4 (SH3RF1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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