Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,366 results for: pcDNA3.1+

Showing: 1651 - 1680 of 5366 results

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pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3

Plasmid

#233062

Purpose

Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

Alfa and HiBiT

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt

Plasmid

#233240

Purpose

Parallel coiled-coil with (GGS)3 linker to prone condensation

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-clover-GCN4-(GGS)3-Vt

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt

Plasmid

#233241

Purpose

Parallel coiled-coil with (GGS)12 linker to prevent condensation

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-clover-GCN4-(GGS)12-Vt

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FFSS-BACH1 C122E

Plasmid

#232274

Purpose

Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH1 (BACH1 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

changed cysteine 122 to glutamic acid

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-Jarid2-polyA

Plasmid

#223811

Purpose

IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

Depositor

Azusa Inoue

Article

Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

Insert

Jarid2 (Jarid2 Mouse)

Use

In vitro transcription

Tags

Flag

Expression

Mammalian

Promoter

CMV, T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-AP4-cyto

Plasmid

#229694

Purpose

Transient expression of EGFP AP4-cyto in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP; Control construct with tandem AP4 motifs (cytoplasm)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-EGFP-FP4-cyto

Plasmid

#229695

Purpose

Transient expression of FP4-cyto in mammalian cells

Depositor

Alpha Yap

Article

Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

Insert

EGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:FRB:gs10:Cul1(dC501)

Plasmid

#228533

Purpose

A fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:FRB:gs10:Cul1(dC501) (CUL1 Human, Synthetic)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:Rbx1:gs10:FRB:HA

Plasmid

#228534

Purpose

Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:Rbx1:gs10:FRB:HA (RBX1 Human, Synthetic)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-8D2Q.Tau2N4R-WPRE

Plasmid

#226382

Purpose

Expresses tau propagation ORF with V5 tag on 8D2Q mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

8D2Q.Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-20D3Q.Tau2N4R-WPRE

Plasmid

#226383

Purpose

Expresses tau propagation ORF with V5 tag on 20D3Q mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

20D3Q, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-8A2R.Tau2N4R-WPRE

Plasmid

#226384

Purpose

Expresses tau propagation ORF with V5 tag on 8A2R mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

8A2R, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-20A3R.Tau2N4R-WPRE

Plasmid

#226385

Purpose

Expresses tau propagation ORF with V5 tag on 20A3R mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

20A3R, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-K281Q.Tau2N4R-WPRE

Plasmid

#226386

Purpose

Expresses tau propagation ORF with V5 tag on K281Q mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

K281Q, 2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-K353Q.Tau2N4R-WPRE

Plasmid

#226387

Purpose

Expresses tau propagation ORF with V5 tag on K353Q mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

K353Q, 2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-S198D.Tau2N4R-WPRE

Plasmid

#226388

Purpose

Expresses tau propagation ORF with V5 tag on S189D mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

S198D.Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-S199D.Tau2N4R-WPRE

Plasmid

#226389

Purpose

Expresses tau propagation ORF with V5 tag on S199D mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T-2A-V5

Expression

Mammalian

Mutation

S199D, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-S202D.Tau2N4R-WPRE

Plasmid

#226390

Purpose

Expresses tau propagation ORF with V5 tag on S202D mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

S202D, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-).TagRFP.T-2A-V5-S404D.Tau2N4R-WPRE

Plasmid

#226391

Purpose

Expresses tau propagation ORF with V5 tag on S404D mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

S404D, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-T217D.Tau2N4R-WPRE

Plasmid

#226392

Purpose

Expresses tau propagation ORF with V5 tag on T217D mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

T217D, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-T403D.Tau2N4R-WPRE

Plasmid

#226393

Purpose

Expresses tau propagation ORF with V5 tag on T403D mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

T403D, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-T181D.Tau2N4R-WPRE

Plasmid

#226394

Purpose

Expresses tau propagation ORF with V5 tag on T181D mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

T181D, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-).TagRFP.T-2A-V5-T231D.Tau2N4R-WPRE

Plasmid

#226395

Purpose

Expresses tau propagation ORF with V5 tag on T231D mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

T231D, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AntiHer2-LC122-TAG

Plasmid

#226839

Purpose

Contains trastuzumab with a TAG codon at position 122 of the light chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 122 of light chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-AntiHer2-HC121-TAG

Plasmid

#226836

Purpose

Contains trastuzumab with a TAG codon at position 121 of the heavy chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 121 of heavy chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-AntiHer2-HC197-TAG

Plasmid

#226838

Purpose

Contains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 197 of heavy chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)

Plasmid

#187727

Purpose

Express his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 226-382 removed (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 226-382

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)

Plasmid

#187728

Purpose

Express his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 418-495 removed (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 418-495

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-WT

Plasmid

#215897

Purpose

Expression of TNRC6A-silencing domain (SD)

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-CAMK2D

Plasmid

#221686

Purpose

Mammalian expression of Myc-tagged BirA*-CAMK2D fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

CAMK2D (CAMK2D Human)

Expression

Mammalian

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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