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Showing: 2201 - 2240 of 8319 results

  1. pENTR CMVie-Lifeact-mTagBFP2 L1-R5

    Plasmid
    #84388
    Purpose
    Multisite Gateway entry vector for expression of mTagBFP2-tagged Lifeact in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Gateway
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV immediate early
    Available since
    Jan. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    84388-plasmid-map-sequence-id-169692

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  2. pET28a SUMO Nup53xl 162-267

    Plasmid
    #86002
    Purpose
    bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53
    Depositor
    Wolfram Antonin
    Type
    Empty backbone
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    86002-plasmid-map-sequence-id-171435

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  3. pMZ377

    Plasmid
    #74214
    Purpose
    Combined sgRNA/Cas9 vector with LEU2 marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74214-plasmid-map-sequence-id-141045

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  4. mEos4b-MKS1-N-18

    Plasmid
    #62776
    Purpose
    Localization: Cilium, N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Depositor
    Loren Looger, Michael Davidson
    Insert
    MKS1
    Use
    Tags
    mEos4b
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pIC215

    Plasmid
    #69736
    Purpose
    Mammalian Expression of PELP1
    Depositor
    Iain Cheeseman
    Insert
    PELP1 (PELP1 Human)
    Use
    Tags
    eYFP-TEV-S
    Expression
    Mammalian
    Mutation
    E927G compared to GenBank reference sequence NP_0…
    Promoter
    Available since
    Dec. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pET15b-At 14-3-3 omicron

    Plasmid
    #44926
    Depositor
    Robert Ferl
    Insert
    14-3-3 omicron (GRF11 Mustard Weed)
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 14, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pIC214

    Plasmid
    #69735
    Purpose
    Mammalian Expression of Tousled-like kinase 1 (TLK1)
    Depositor
    Iain Cheeseman
    Insert
    Tousled-like kinase 1 (TLK1) (TLK1 Human)
    Use
    Tags
    eYFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pET15b-Fab1 (S. aureus)

    Plasmid
    #61691
    Purpose
    Inducible expression of the FabI enzyme from S. aureus
    Depositor
    Michael Johnson
    Insert
    FabI
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pJK554

    Plasmid
    #72453
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72453-plasmid-map-sequence-id-133893

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  10. mTagBFP2-TES-N-14

    Plasmid
    #62786
    Purpose
    Localization: Focal Adhesions, N1 Cloning Vector, Excitation:399, Emission:456
    Depositor
    Michael Davidson
    Insert
    TES (TES Human)
    Use
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62786-plasmid-map-sequence-id-173171

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  11. pET15b-ACP (from S. aureus)

    Plasmid
    #63686
    Purpose
    Inducible expression of Acyl Carrier Protein (ACP) from S. aureus
    Depositor
    Michael Johnson
    Insert
    ACP
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized
    Promoter
    T7
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pET-sfGFP-GGS9-Cre-6xHis

    Plasmid
    #89343
    Purpose
    Expresses sfGFP fused to Cre in E.Coli
    Depositor
    David Liu
    Insert
    sf-GFP-GGS9-Cre-6xHis
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    89343-plasmid-map-sequence-id-176159

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  13. mEos-A69V-C1

    Plasmid
    #54647
    Purpose
    Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mEos A69V
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pNIC28-Bsa4-iLOVf

    Plasmid
    #63722
    Purpose
    LIC E coli expression plasmid to produce TEV cleavable His tagged iLOV fusion proteins
    Depositor
    Brian Smith
    Type
    Empty backbone
    Use
    Tags
    TEE, iLOV, 6xHis, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63722-plasmid-map-sequence-id-106345

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  15. pTUB:FLuc

    Plasmid
    #114578
    Purpose
    Constitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integration
    Depositor
    David Sibley
    Insert
    Firefly Luciferase
    Use
    Cre/Lox and Luciferase
    Tags
    Expression
    Mutation
    Promoter
    alpha tubulin T. gondii
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    114578-plasmid-map-sequence-id-220131

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  16. mEmerlad-MCPB2-C-18

    Plasmid
    #62767
    Purpose
    Localization: Actin Capping Protein , C1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    MCPB2
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62767-plasmid-map-sequence-id-235388

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  17. pJK694

    Plasmid
    #72379
    Purpose
    Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    phosphoribosyl pyrophosphate amidotransferase (PPAT Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GPD
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pHEX2 T494 TALENs

    Plasmid
    #44464
    Depositor
    Roger Hellens, Ross Johnson
    Insert
    AtCRU3 T494 TALENs
    Use
    Plant transformation
    Tags
    Expression
    Mutation
    Promoter
    CaMV 35S
    Available since
    May 6, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pFA6a-SRM (sortase recognition motif)-hphMX4

    Plasmid
    #54488
    Purpose
    PCR based C-terminal SRM tagging in yeast
    Depositor
    Eri Sakata
    Insert
    SRM (sortase-recognition motif)
    Use
    Yeast genome targeting
    Tags
    hphMX4
    Expression
    Mutation
    Promoter
    Available since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pKPY738

    Plasmid
    #72674
    Purpose
    C. elegans let-858 3' UTR (Gateway Entry Clone)
    Depositor
    David Tirrell
    Insert
    let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    72674-plasmid-map-sequence-id-133995

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  21. mGeos2-VEL-C1

    Plasmid
    #54541
    Purpose
    Localization: C1 Cloning Vector, Excitation: 503 / 569, Emission: 514 / 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mGeos2-VEL
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pFA6a-kanMX-tetO-Pcyc1-GST-LINKER

    Plasmid
    #52688
    Purpose
    Same as 41026, with additional linker
    Depositor
    Michael Nick Boddy
    Insert
    tetO7-Pcyc1-GST+LINKER
    Use
    Promoter replacement
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    52688-plasmid-map-sequence-id-82018

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  23. pAG Lenti CMV HA-EGFP Puro

    Plasmid
    #236081
    Purpose
    Lentiviral backbone expressing N-terminally HA tagged EGFP, control for empty backbone 236079
    Depositor
    Melina Fan
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236081-plasmid-map-sequence-id-467821

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  24. Ec holD-pET(3c)

    Plasmid
    #237236
    Purpose
    over-express E. coli holD (Pol III psi subunit)
    Depositor
    Michael O'Donnell
    Insert
    holD (psi) (holD E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237236-plasmid-map-sequence-id-469263

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  25. Ec holC-pET(3c)

    Plasmid
    #237235
    Purpose
    over-express E. coli holC (Pol III chi subunit)
    Depositor
    Michael O'Donnell
    Insert
    holC (chi) (holC E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237235-plasmid-map-sequence-id-469249

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  26. pCAGGS-MTS(S23A)-EGFP-V5

    Plasmid
    #200929
    Purpose
    expresses MTS(S23A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    MTS amino acid 23 mutated to Alanine
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200929-plasmid-map-sequence-id-410100

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  27. pCAGGS-MTS(S6A)-EGFP-V5

    Plasmid
    #200817
    Purpose
    expresses MTS(S6A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    Changed Serine 6 to Alanine in MTS
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200817-plasmid-map-sequence-id-409561

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  28. YFP Rab11 Q70L in pcDNA3.1

    Plasmid
    #90467
    Purpose
    RhoA mediated cell migration
    Depositor
    Narasimhan Gautam
    Insert
    Rab11 Q70L
    Use
    Tags
    mEYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    90467-plasmid-map-sequence-id-197844

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  29. pCCI-YFVΔCMENS1-Flpo

    Plasmid
    #233592
    Purpose
    pCCI vector encoding YFV-17D lacking C, prM, E and NS1 proteins but with Flpo
    Depositor
    Wei Xu
    Insert
    YFV-17D lacking C, prM, E and NS1 proteins but with Flpo
    Use
    Transneural tracing
    Tags
    Expression
    Mutation
    Lacking C, prM, E and NS1
    Promoter
    SP6
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233592-plasmid-map-sequence-id-467449

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  30. pZP06B

    Plasmid
    #232321
    Purpose
    Make sacB a counterselection marker to replace galK
    Depositor
    Chenggang Wu
    Insert
    PrpsJ-sacB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232321-plasmid-map-sequence-id-462085

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  31. pET-Impact-T4gp41

    Plasmid
    #228207
    Purpose
    Expression in BL21(DE3) of T4 helicase
    Depositor
    Stephen Benkovic
    Insert
    41 (41 Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228207-plasmid-map-sequence-id-453945

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  32. pET-Impact-T4gp59

    Plasmid
    #228206
    Purpose
    Expression in BL21(DE3) of WT T4 helicase loader
    Depositor
    Stephen Benkovic
    Insert
    59 (59 Escherichia phage T4)
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228206-plasmid-map-sequence-id-453944

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  33. Pv6PGDH-pNic-Bsa4

    Plasmid
    #231102
    Purpose
    Expresses Plasmodium vivax 6-phosphogluconate dehydrogeanse under T7 with HIS tag
    Depositor
    Josh Beckham
    Insert
    6-phosphogluconate dehydrogenase
    Use
    Tags
    HIS tag
    Expression
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231102-plasmid-map-sequence-id-462632

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  34. 1203D-5'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid
    #221534
    Purpose
    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' end, 22nt mCherry-targeting spacer in between DRs
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221534-plasmid-map-sequence-id-437444

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  35. 1203E-3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid
    #221535
    Purpose
    EGFP reporter construct with double unprocessed direct repeat (35nt) at 3' end, 22nt mCherry-targeting spacer in between DRs
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221535-plasmid-map-sequence-id-443095

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  36. 1203F-5’+3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid
    #221536
    Purpose
    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' and 3' end, 22nt mCherry-targeting spacer in between DRs
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221536-plasmid-map-sequence-id-437446

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  37. 1203AB-CMV-Cas7.1-H43A-mutant

    Plasmid
    #221541
    Purpose
    Effector expresses Cas7.1 (truncated from DiCas7-11), H to A mutation at amino acid 43
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221541-plasmid-map-sequence-id-437821

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  38. 1203AC-CMV-Cas7.1-Y55A-mutant

    Plasmid
    #221542
    Purpose
    Effector expresses Cas7.1 domain (truncated from DiCas7-11), Y to A mutation at amino acid 55
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221542-plasmid-map-sequence-id-437820

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  39. pGLO-Yellow-AZA

    Plasmid
    #220783
    Purpose
    Expresses a yellow chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)
    Depositor
    Angela Leu
    Insert
    yellow chromoprotein
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    220783-plasmid-map-sequence-id-444833

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  40. MZB174

    Plasmid
    #228487
    Purpose
    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    10xHis-SUMO(Smt3)-GST-HRV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228487-plasmid-map-sequence-id-455394

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Showing: 2201 - 2240 of 8319 results