We narrowed to 10,632 results for: t7

1. pCS4

   Plasmid

   #55751

   Purpose

   Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Mutation

   Residues 1-59 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-HsSENP5

   Plasmid

   #71468

   Purpose

   Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 5 (SENP5 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCS5

   Plasmid

   #55750

   Purpose

   Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Mutation

   Residues 59-264 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET23a-MmRPS27a

   Plasmid

   #69559

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   RPS27A (RPS27A Mouse, Human)
   

   Expression

   Bacterial

   Mutation

   Changed Alanine 133 (human RPS27a) to Glycine (mo…

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pETM6-G6-mCherry

   Plasmid

   #66533

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter G6

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - G6

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-H9-mCherry

   Plasmid

   #66532

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter H9

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - H9

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEU3-NII-HLICNot

   Plasmid

   #140181

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 6x His-tag and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   pEU3-NII-HLICNot

   Promoter

   T7

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a.MJ1247

   Plasmid

   #11414

   Depositor

   Sung-Hou Kim

   Article

   Martinez-Cruz et al Structure. 2002 Feb . 10(2):195-204.

   Insert

   3-hexulose-6-phosphate isomerase (MJ_1247 M. jannaschii)
   

   Tags

   T7-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. ED_p_060

   Plasmid

   #246070

   Purpose

   ECOR31 mCpol bacterial expression under T7 promoter, C-term TEV-MBP-HIS10

   Depositor

   Jennifer Doudna

   Article

   Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

   Insert

   Minimal CRISPR polymerase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. ED_p_044a

    Plasmid

    #246071

    Purpose

    ECOR31 mCpol bacterial expression under T7 promoter, N-term His10-MBP-TEV

    Depositor

    Jennifer Doudna

    Article

    Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

    Insert

    Minimal CRISPR polymerase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19_21 tRNA genes

    Plasmid

    #242430

    Purpose

    In vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.

    Depositor

    Sebastian Maerkl

    Article

    Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

    Insert

    21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene
    

    Expression

    Bacterial

    Available Since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. SMT503

    Plasmid

    #226840

    Purpose

    Plasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramp

    Depositor

    Polly Fordyce

    Article

    Thompson et al Adv Mater Interfaces 2024, 12 (3), 24004

    Insert

    Lipase A
    

    Use

    Synthetic Biology

    Tags

    SNAP and eGFP

    Expression

    Bacterial

    Mutation

    R33Q, D34N, K35D, K112D, M134D, Y139C, I157M

    Promoter

    T7

    Available Since

    Aug. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

    Plasmid

    #239294

    Purpose

    Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

    Depositor

    Anita Hopper

    Article

    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

    Inserts

    T7 promoter
    ITS-tRNAIle intron
    T7 terminator - Var1
    

    Expression

    Yeast

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. small ubiquitin-like modifier 3 (SUMO3)

    Plasmid

    #221053

    Purpose

    Expression of small ubiquitin-like modifier 3 (SUMO3) protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    SUMO 3 (SUMO3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-2H12Matry

    Plasmid

    #221187

    Purpose

    His6-tagged cdGreen-Matry expressed from T7 promoter (Internal Lab ID: UJ11309)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen-Matry
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28b-TEV-AcrIC9

    Plasmid

    #207409

    Purpose

    Protein purification vector to produce AcrIC9 in E. coli

    Depositor

    Yan Zhang

    Article

    Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

    Insert

    T7-TEV-AcrIC9
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet-3C-AcrIC8

    Plasmid

    #207410

    Purpose

    Protein purification vector to produce AcrIC8 in E. coli

    Depositor

    Yan Zhang

    Article

    Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

    Insert

    T7-HRV3C-AcrIC8
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDL28_Cas9-His Δcys E532C/E1207C

    Plasmid

    #206289

    Purpose

    Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S, E1207C

    Promoter

    T7

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDL27_Cas9-His Δcys E532C/E945C

    Plasmid

    #206288

    Purpose

    Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S, E945C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDL26_Cas9-His Δcys M1C/E532C

    Plasmid

    #206287

    Purpose

    Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    M1C, C80S, E532C, C574S

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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