We narrowed to 11,594 results for: EMB
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Plasmid#127406PurposeExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsFLAGExpressionMammalianMutationAmino acid mutations: E322 to Amber codon, K305H,…PromoterCMVAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-K295TAG-W340A-S233H-T237C.pcDNA3-k
Plasmid#127402PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsFLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-K295TAG-W340A-S233H-T237H.pcDNA3-k
Plasmid#127400PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsFLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 delta69-97
Plasmid#124924PurposeFor expression of a.a.69-97 deletion mutant of SIN1-GFPDepositorAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2-6Gly-sfGFP
Plasmid#124065PurposeE. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.DepositorInsertcytATL-sfGFP
TagssfGFPExpressionBacterialPromotertrcAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB026
Plasmid#119711PurposeTranscriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMTDepositorInsertPromoter gpdA:YFP coding sequence:Terminator trpC
UseSynthetic BiologyAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP V107M-L273D-L276D
Plasmid#114183PurposeORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
TagsYFPExpressionMammalianMutationV107M/L273D/L276DAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP T184M-L273D-L276D
Plasmid#114184PurposeORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
TagsYFPExpressionMammalianMutationT184M/L273D/L276DAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q8DTE3
Plasmid#103129PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ8DTE3(Cas9 coding gene from Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-D3UFL8
Plasmid#103095PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertD3UFL8(Cas9 coding gene from Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/181/666E
Plasmid#105314PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2-N[80]A
Plasmid#96995PurposeExpress GFP-tagged mutated mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
TagsGFPExpressionPlantMutationAmino acid residue 80 was mutated (N[80]A). Mutat…Promoter35SAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD1cR
Plasmid#86970PurposeExpresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coliDepositorInsertPCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, G108C in PCNA1PromoterT7 promoterAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT
Plasmid#61789PurposeLuciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutatedDepositorInsertmouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
UseLuciferaseMutationboth miR-200c MRE-2 and MRE-3 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-mOC-STAMP (N162D)-GFP
Plasmid#73580PurposeLentivirus expression of mOC-STAMP with N162D mutationDepositorInsertOC-STAMP (Ocstamp Mouse)
UseLentiviralTagsGFPMutationchanged asparagin (N) 162 to Aspartic Acid (D)Available SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Jam4.3-Fc-His
Plasmid#72082PurposeExpresses the extracellular region of the JAM-4, isoform 3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Jam4.1-AP-His
Plasmid#71955PurposeExpresses the extracellular region of the JAM-4, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Jam4.3-AP-His
Plasmid#71956PurposeExpresses the extracellular region of the JAM-4, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S279A
Plasmid#60705Purposeretroviral expression of rat Cx43 S279A mutantDepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1412
Plasmid#63787PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection markerDepositorInserteCitrine-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseCitrine-Strep-tag IIPromoternoneAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only