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EC67845

Plasmid

#209471

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Anep8_Cas9

Plasmid

#117169

Purpose

Expressed Cas9 in Aspergillus with LIC tags for easy gRNA cloning

Depositor

Adrian Tsang

Article

Song et al PLoS One. 2018 Aug 24;13(8):e0202868. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Filamentous fungi e…

Promoter

Pki

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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LaM8-GG15-irFP

Plasmid

#159591

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at GG15

Use

Lentiviral

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCMU-APr

Plasmid

#61182

Purpose

Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-dCas9-GFP

Plasmid

#64104

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

EFS

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT58_X2site

Plasmid

#177705

Purpose

Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pPM2213

Plasmid

#167553

Purpose

Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

Depositor

Renato Morona

Article

Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

Insert

rfb region of Shigella flexneri serotype 2a

Expression

Bacterial

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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EC70191

Plasmid

#209452

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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HLA_B5101

Plasmid

#165011

Purpose

Expression of HLA-B*51:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-B*51:01 (HLA-B Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-talin1 head

Plasmid

#32856

Depositor

Anna Huttenlocher

Article

Simonson et al J Immunol. 2006 Dec 1;177(11):7707-14.

Insert

Talin-1 Head aa 1-433 (Tln1 Mouse)

Tags

Enhanced GFP

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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EC70204

Plasmid

#209465

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pME-minP:luc2

Plasmid

#138354

Purpose

Gateway compatible middle entry clone contains Firefly Luciferase2 insert driven by minimal promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

Luciferase

Expression

Mammalian

Promoter

minimal promoter

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFsynW TeTx-LC

Plasmid

#197284

Purpose

Encodes for viral expression of the light chain of tetanus toxin.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

Tetanus toxin light chain

Use

Lentiviral

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1345 mCherry-FIREtag-DEVDG-MoA

Plasmid

#205176

Purpose

Expresses mCherry-FIREtag-DEVDG-MoA in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

mCherry-cagedFIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-GFPuv

Plasmid

#127078

Purpose

Low phosphate inducible yibD gene promoter expressing GFPuv reporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-CMVSP6

Plasmid

#138356

Purpose

Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

CMV-SP6 promoter

Use

Gateway compatible

Promoter

CMV-SP6

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-LacZshRNA

Plasmid

#73978

Purpose

LacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the LacZ gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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EC70206

Plasmid

#209467

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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sfTq2ox-N1

Plasmid

#117930

Purpose

Superfolder mTurquoise2 ox (pNM113)

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2ox

Expression

Mammalian

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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ttAtCas12a+int

Plasmid

#182384

Purpose

Gives very high editing efficiency in barley. Also works in Brassica oleracea

Depositor

Wendy Harwood

Article

Lawrenson et al bioRxiv 2022.04.28.489853

Insert

LbCas12a coding sequence with D156R and Arabidopsis introns

Use

CRISPR

Mutation

D156R + 8 Arabidopsis introns added

Promoter

None

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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