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pET21a(+)_co_L-HicDH

Plasmid

#185453

Purpose

CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

Depositor

Wolfgang Kroutil

Article

Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

Insert

L-HicDH from Lactobacillus confusus DSM 20196

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Arkadia C946S H948L

Plasmid

#180373

Purpose

expressing mouse Arkadia (isoform 3) C946S H948L E3 ligase dead mutant proteins

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia (Rnf111 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

C946S H948L

Promoter

LTR promoter

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2108

Plasmid

#179252

Purpose

Punc-4::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Tags

wCherry

Expression

Worm

Mutation

M280I

Promoter

Punc-4

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2106

Plasmid

#179250

Purpose

Pnmr-1::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pnmr-1

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-gltA1-gltA2

Plasmid

#71348

Purpose

gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

gltA1-gltA2 guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-TVA-eGFP-N2cG

Plasmid

#175440

Purpose

Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRD445

Plasmid

#168775

Purpose

Expression of mEos3.2 in Mycobacterium

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mEos3.2

Expression

Bacterial

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-copGFP-Polß(L301R/V303R/V306R)-puro

Plasmid

#128666

Purpose

mammalian expression of copGFP-Polß(L301R/V303R/V306R: TM) protein with puromcyin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM)-IRES-hyg

Plasmid

#128668

Purpose

mammalian expression of Flag-Polß(TM: L301R/V303R/V306R) protein with hygromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-mirMecp2

Plasmid

#163704

Purpose

pAAV plasmid to induce expression of micro-RNA targeted against mouse Mecp2 and EGFP

Depositor

Michisuke Yuzaki

Article

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirMecp2 and EGFP (Mecp2 Mouse, Aequorea victoria)

Use

AAV

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM-K206A)-IRES-puro

Plasmid

#128679

Purpose

mammalian expression of Flag-Polß(TM-K206A: L301R/V303R/V306R-K206A) protein with puromycin slection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R/K206A

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(KTM)-IRES-Neo

Plasmid

#128670

Purpose

mammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K41R/K61R/K81R

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(L301R/V303R/V306R)-IRES-puro

Plasmid

#128657

Purpose

mammalian expression of Flag-Polß(L301R/V303R/V306R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(KTM)-IRES-puro

Plasmid

#128672

Purpose

mammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K41R/K61R/K81R

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM-K244A)-IRES-puro

Plasmid

#128680

Purpose

mammalian expression of Flag-Polß(TM-K244A: L301R/V303R/V306R-K244A) portein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R/K244A

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(TM)-IRES-neo

Plasmid

#128671

Purpose

mammalian expression of Flag-Polß(TM: L301R/V303R/V306R) protein with neomycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM41991

Plasmid

#153219

Purpose

Level M binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Expression

Plant

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-BBclone1

Plasmid

#137069

Purpose

E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Inserts

BB0405 (BB_0405 Borrelia burgdorferi B31)
BB0406
P66
BBA57
HtrA
RevA (revA Borrelia burgdorferi B31)
BB0238

Expression

Bacterial

Mutation

wild-type, full-length (signal-peptide-containing…

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe2-WT

Plasmid

#85845

Purpose

Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 2 homology arms (SNCA Human)

Tags

-

Expression

Bacterial and Mammalian

Mutation

-

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pTW927

Plasmid

#115961

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 ORF TetR-2A-mKate-PEST GG

Use

Synthetic Biology

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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