165,775 results

1. pCS2-mCherry-Cenexin

   Plasmid

   #186435

   Purpose

   Expresses mCherry Cenexin in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al Mol Biol Cell. 2022 Aug 1;33(9):br14. do

   Insert

   Cenexin (ODF2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLC242-GFP-metap2

   Plasmid

   #100518

   Purpose

   Expresses GFP tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   Metap2 (METAP2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-U6gRNA1-U6gRNA2-TnT-Cre

   Plasmid

   #87682

   Purpose

   AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

   Depositor

   William Pu

   Article

   Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

   Inserts

   gRNA1
   gRNA2
   Cre
   

   Use

   AAV

   Promoter

   U6 and cTnT

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. opto-RhoA-mCherry_pcDNA3.1

   Plasmid

   #164472

   Purpose

   Expresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

   Insert

   opto-RhoA
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. scFv-GCN4-sfGFP-TET1CD

   Plasmid

   #184439

   Purpose

   TET1 catalytic domain fused with sfGFP and scFv against GCN4

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   scFv-GCN4-sfGFP-TET1CD
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. XRE-pNL1.3[secNLuc]

   Plasmid

   #182295

   Purpose

   Secreted Luciferase reporter for AhR activity

   Depositor

   Severine Degrelle, Thierry Fournier

   Article

   Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

   Insert

   Xenobiotic Response Element (CYP1A1 Human)
   

   Tags

   secNluc

   Expression

   Mammalian

   Promoter

   XRE

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Dcp1b

   Plasmid

   #153976

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1b

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA recapping enzybe 1b (DCP1B Human)
   

   Tags

   pAcGFP-C1

   Expression

   Mammalian

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV Ef1a-EGFP-WPRE

   Plasmid

   #135428

   Purpose

   Can be used to express EGFP. Can also be used to create adeno-associated virus for delivery of the EGFP sequence

   Depositor

   Rylan Larsen

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL1425 (pSPIN, pCDF backbone)

   Plasmid

   #160735

   Purpose

   Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBroad-T7RNAP-apraR

    Plasmid

    #199993

    Purpose

    T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    T7RNAP Circuit
    

    Expression

    Bacterial

    Mutation

    copFnull

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-IRAlus-Nicn1

    Plasmid

    #92345

    Purpose

    Plasmid for expression of GFP gene with a Nicn1 gene derived IR Alu element containing 3'UTR.

    Depositor

    Gordon Carmichael, Ling-Ling Chen

    Article

    Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

    Insert

    Nicn1 gene derived IR Alu element containing 3'UTR (NICN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET His6 Thioredoxin TEV LIC cloning vector (2T-T)

    Plasmid

    #29712

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6-Thioredoxin-TEV

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-flag YAP2 5SA

    Plasmid

    #27371

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP2 (YAP1 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Serines at positions 61, 109, 127, 128, 131, 163,…

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. C1(1-29)-miniTurbo-V5_pLX304

    Plasmid

    #107176

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Lentiviral

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. GluN2A (px330:2-guideCas9)

    Plasmid

    #169428

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriExRhoA2G

    Plasmid

    #40176

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB_TRE_Z18-PDGFR_EF1a_eGFP

    Plasmid

    #186304

    Purpose

    Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    Z18-PDGFR
    eGFP
    

    Tags

    HA, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-HA-USP36

    Plasmid

    #22579

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP36 (USP36 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI-SEP-GluR1

    Plasmid

    #24000

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    GluR1 (Gria1 Rat)
    

    Tags

    GluR1 signal peptide (aa1-22) and SEP (supereclip…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF3167

    Plasmid

    #144643

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF512B (ZNF512B Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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