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Plasmid#112614Purposepromoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2DepositorInsertsUsePromoterless vector, ready for promoter for mamma…TagsVenusExpressionMammalianPromoterNo PromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only
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ptdTHC_PGKneoLox2DTA.2
Plasmid#112625PurposeH2BtdTomato_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology armsDepositorInsertsUseCre/Lox and Mouse TargetingTagstdTomatoPromoterNo PromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron
Plasmid#246435PurposeExpresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS.DepositorInsertsO.gue IscB with dead mutations (D60A, H269A) and delta-TID
EGFP
TagsSV40 NLS, Thosea asigna virus 2A peptide, and nuc…ExpressionMammalianMutationchanged Aspartic Acid 60 to Alanine, changed Hist…PromoterCMVAvailable sinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
MB 45t3 thsS(t3)R-sfGFP_mCherry
Plasmid#232470PurposeOptimized thiosulfate sensor with fluorescent reporter (GFP), constitutive mCherryDepositorInsertsthsS(t3)
thsR
PphsA342
sfGFP
AxeTxe
mCherry
ExpressionBacterialMutationContains the following mutations K286Q, Q350H, I…PromoterJ23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…Available sinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)
Plasmid#208291PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations and V106W in TadA and nSpRY(D10A/…PromoterCMV and T7Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605
Plasmid#108362PurposeExpression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegansDepositorInsertsTags2xFlag, MYC, T2A peptide, and TY1 linkerExpressionWormPromoterA rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…Available sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8H.28TM.BB.3z
Plasmid#200675PurposeModular CD8hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196082Purpose(Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Aminoglycoside phosphotransferase
UseCRISPRExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8H.28TM.BB.28.3z
Plasmid#200677PurposeModular CD8hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SeqCBE
Plasmid#232115PurposeExpresses AncBE4max, nSeqCas9 (D10A), and puromycin resistance genesDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB117
Plasmid#228481Purpose"Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)DepositorTypeEmpty backboneTagsGST (glutathione S-transferase)ExpressionBacterialPromoterT7 PromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPF4213
Plasmid#254224PurposeMutagenesis plasmid encoding tnpA and the tn5 transposon harbouring AcrVIA1DepositorInsertAcrVIA1
UseTransposon insertion mutagenesis vectorMutationCodon optimized for E. coli.Promoterjs23100Available sinceJuly 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGLU_11
Plasmid#225819PurposeTn libraries Himar variant CAM backboneDepositorPublicationTypeEmpty backboneUseSynthetic BiologyMutationN/AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
hIgG1-G6-Fc
Plasmid#194594PurposeMammalian expression of G6 nanobody upstream of human IgG1; G6 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertG6-Fc (S )
ExpressionMammalianAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
hU6-Sa_tracr
Plasmid#135973PurposeContains tracrRNA-hU6 sequence for double sgRNA cloningDepositorTypeEmpty backboneExpressionMammalianAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
C86m
Plasmid#120997PurposeMoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.DepositorInsertX174E
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C24m
Plasmid#120964PurposeMoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version). Please see Supplemental Documents for annotated Genbank file.DepositorInsertlambda-cI
UseSynthetic BiologyAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mCherry
Plasmid#223956PurposeGateway cloning of genes of interest with an N-terminal V5-mCherry fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mCherryAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_mNeonGreen_FLAG_C
Plasmid#218073PurposeRESOLUTE cloning plasmidDepositorTypeEmpty backboneUseLentiviralTagsFLAG-mNeonGreen-V5Available sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMal-mRuby3
Plasmid#209348PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal mRuby3-StepTagIIDepositorTypeEmpty backboneExpressionBacterialAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only