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CMV-HA-hTAF5

Plasmid

#17984

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF5 (TAF5 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
Human NCKX6 (pcDNA3.1+)

Plasmid

#75216

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Article

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (SLC8B1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ndel1

Plasmid

#12571

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Ndel1 (NDEL1 Human)

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
ABE-RA2.1

Plasmid

#208300

Purpose

Expresses ABE-RA2.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA2.1-SpCas9 D10A

Expression

Mammalian

Mutation

P48A, R51H, D108G, K110R, H122R, M126I, N127K

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pICH41414

Plasmid

#50337

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCM4.4

Plasmid

#217648

Purpose

an ermE*p-cas9-driven expression, as an alternative plasmid

Depositor

Huimin Zhao

Article

Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

Insert

Codon Optimized SpCas9

Use

CRISPR

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS

Plasmid

#19446

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HIS

Expression

Mammalian

Mutation

HIS tag inserted to create new vector

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-NanoLuc-PuroR

Plasmid

#80962

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

NanoLuc

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pHLU

Plasmid

#64167

Purpose

harbours marker genes HIS3, LEU2 and URA3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGL193

Plasmid

#180471

Purpose

For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

Depositor

Peggy Lemaux

Article

Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Cas9

Expression

Plant

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFH35

Plasmid

#126009

Purpose

Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCRL1

Plasmid

#153997

Purpose

sgRNA in vitro transcription plasmid.

Depositor

Shengdar Tsai

Article

Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

Type

Empty backbone

Use

CRISPR; Sgrna under a t7 promoter for in vitro tr…

Promoter

T7

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Sapphire

Plasmid

#96950

Purpose

Retroviral plasmid containing Sapphire for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Sapphire fluorescent protein

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Lis1

Plasmid

#12574

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Lis1 (Pafah1b1 Mouse)

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pSKB-

Plasmid

#62540

Purpose

Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Bacterial

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pmTurquoise-N1

Plasmid

#60559

Purpose

Expresses the cyan fluorescent protein variant mTurquoise

Depositor

Dorus Gadella

Article

Goedhart et al Nat Methods. 2010 Feb;7(2):137-9. doi: 1

Insert

mTurquoise

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pSA293-CHEQ-seq

Plasmid

#174669

Purpose

Reporter plasmid for MPRA containing a SCP1 promoter, a chimeric intron and the Venus cDNA.

Depositor

Stein Aerts

Article

Mauduit et al Elife. 2021 Dec 7;10. pii: 71735. doi: 1

Type

Empty backbone

Use

Enhancer reporter

Promoter

SCP1

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN51 (R40A)

Plasmid

#80109

Purpose

Late1-O3-33-myc-PH(R40A)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51 (R40A)

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pPd4E-ISH

Plasmid

#61700

Purpose

Plum eIF4E ihp-RNA construct

Depositor

Lining Tian

Article

Wang et al PLoS One. 2013;8(1):e50627. doi: 10.1371

Insert

PdeIF4E

Use

RNAi

Promoter

35S

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pYC1876

Plasmid

#158722

Purpose

Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

ssrA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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