We narrowed to 37,507 results for: Ran;

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Cherry-ICP0FxE-C3

Plasmid

#134562

Purpose

Expresses Cherry-ICP0 fusion protein with a RING finger deletion (FXE)

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

ICP0

Tags

Cherry

Expression

Mammalian

Mutation

RING finger deletion (FXE)

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJM160

Plasmid

#134169

Purpose

Bacterial expression of RB3 stathmin-like domain (SLD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

RB3-SLD (STMN4 Human)

Expression

Bacterial

Mutation

residues 49-189

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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px330-CD4

Plasmid

#136938

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting CD4 (CD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLRC1-NEK10p:NEK10-3XFLAG

Plasmid

#137030

Purpose

Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

Depositor

David Sabatini

Article

Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

Insert

NIMA-like kinase 10 (NEK10 Human)

Use

Lentiviral

Tags

3XFLAG

Promoter

human NEK10 endogenous promoter

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW-EGFP

Plasmid

#133400

Purpose

Piggybac vector for constitutive expression of EGFP

Depositor

Blair Madison

Article

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

EGFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF64-2xNSTREP

Plasmid

#136224

Purpose

Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF64

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF26-2xCSTREP

Plasmid

#136186

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF26

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF26

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF52-2xCSTREP

Plasmid

#136212

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF52

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF52

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

Plasmid

#130976

Purpose

Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

Depositor

Anne Straube

Article

Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

Insert

Hook3-SNAPf (HOOK3 Human)

Tags

8His-ZZ-LTLT and SNAPf

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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ELK4_pet28a

Plasmid

#131648

Purpose

Bacterial expression of ETS gene ELK4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK4 (ELK4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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B4GALT1-Dox

Plasmid

#124633

Purpose

Doxycycline-inducible B4GALT1 expression in mammalian cells

Depositor

Ron Weiss

Article

Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

Inserts

Beta-(1,4)-Galactosyltransferase 1
Tetracycline repressor A3 mutant
TagFP635

Expression

Mammalian

Promoter

TRE-tight and hEF1a

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pESR1-donor

Plasmid

#112337

Purpose

CRISPR donor plasmid to tag human transcription factor ESR1 with GFP

Depositor

Kevin White

Insert

ESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)

Use

CRISPR

Mutation

Unknown

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT/Smarcc1

Plasmid

#122145

Purpose

For making Smarcc1 RNA

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Smarcc1 (Smarcc1 Mouse)

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pESR1.1.0-gDNA

Plasmid

#112433

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1

Depositor

Kevin White

Insert

ESR1 (ESR1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Col2.3deltaTK

Plasmid

#114273

Purpose

Conditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.

Depositor

David Rowe

Article

Visnjic et al J Bone Miner Res. 2001 Dec;16(12):2222-3

Insert

Col2.3 promoter + deltaTK + BGH polyA

Expression

Mammalian

Promoter

2.3 kb rat type 1 collagen

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAH36 (Pmyo-2::cGAL-C::let-858 3'UTR)

Plasmid

#107135

Purpose

cGAL-C driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Expression

Worm

Promoter

C. elegans myo-2 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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BCL2-pcw107-V5

Plasmid

#64630

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

BCL2 (transcript variant alpha) (BCL2 Human)

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
sgRNA3_MYOD1

Plasmid

#64138

Purpose

Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_MYOD1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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mRuby2-SiT-N-15

Plasmid

#55912

Purpose

Localization: Golgi TGN, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

SiT (ST6GAL1 Human)

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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