We narrowed to 570 results for: ZAC

1. pHJ4

   Plasmid

   #158785

   Purpose

   Pan-neuronal nuclear-localized GCaMP expression in C. elegans

   Depositor

   Zachary Knight, Jennifer Garrison

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   Prab-3::nls::GCaMP6m
   

   Use

   Tags

   NLS

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMax_ScaI_BFP

   Plasmid

   #177824

   Purpose

   Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   tagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5' inserted ScaI RE cut site

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GfaABC1D BARK D110A p2A mCherry

   Plasmid

   #117692

   Purpose

   GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   AAV

   Tags

   HA / FLAG

   Expression

   Mutation

   D110A

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

   Plasmid

   #120293

   Purpose

   AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   N-terminal fragment of SpyCas9
   

   Use

   AAV and CRISPR

   Tags

   SV40-NLS and split-intein

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMax_mPlum_C207G/T208C

   Plasmid

   #177831

   Purpose

   Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mPlum_C207G/T208C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C207G + T208C

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. U6-sgRNAs-Crym

   Plasmid

   #200067

   Purpose

   For the knock down of Crym with an astrocyte specific mCherry expression

   Depositor

   Baljit Khakh

   Article

   Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

   Insert

   U6-3xsgRNA-Crym
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   GfaABC1D-mCherry

   Expression

   Mutation

   Promoter

   U6, GfaABC1D

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Circular 200,100 (mPCSK9)

   Plasmid

   #170122

   Purpose

   AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (Pcsk9 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

   Plasmid

   #120294

   Purpose

   AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   C-terminal fragment of SpyCas9
   

   Use

   AAV and CRISPR

   Tags

   split-intein

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

   Plasmid

   #120295

   Purpose

   AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   C-terminal fragment of SpyCas9
   

   Use

   AAV and CRISPR

   Tags

   split-intein

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Circular 200,100 (mIDUA)

    Plasmid

    #170123

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Idua Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge