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We narrowed to 15,661 results for: genome

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Showing: 2241 - 2280 of 15661 results

  3. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  5. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

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  6. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid
    #134923
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134923-plasmid-map-sequence-id-267593

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  8. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

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  9. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid
    #134925
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134925-plasmid-map-sequence-id-267605

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  10. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid
    #134924
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134924-plasmid-map-sequence-id-267600

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  11. pUDP002

    Plasmid
    #103872
    Purpose
    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Inserts
    hph
    Spcas9 D147Y P411T
    Use
    CRISPR
    Tags
    NLS
    Expression
    Yeast
    Mutation
    D147Y P411T
    Promoter
    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103872-plasmid-map-sequence-id-199260

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  15. pLP-PIGA (pLP140)

    Plasmid
    #168461
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.
    Depositor
    Jef Boeke, Matthew Maurano
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    168461-plasmid-map-sequence-id-362577

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  17. pPL001

    Plasmid
    #184750
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    184750-plasmid-map-sequence-id-365374

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  18. pLP-PIGA2 (pLP300)

    Plasmid
    #168462
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.
    Depositor
    Matthew Maurano
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    168462-plasmid-map-sequence-id-362578

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  19. pRL838

    Plasmid
    #70680
    Purpose
    This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.
    Depositor
    Peter Wolk
    Article
    Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53
    Type
    Empty backbone
    Use
    Cre/Lox
    Available Since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70680-plasmid-map-sequence-id-126382

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  29. pP[RS3]3'M

    Plasmid
    #53552
    Purpose
    for an easy screen of TALEN- and CRISPR/Cas9- mediated mutagenesis in Drosophila
    Depositor
    Renjie Jiao
    Article
    Yu et al Biol Open. 2014 Apr 15;3(4):271-80. doi:
    Insert
    AscI and MluI restriction enzyme sites
    Expression
    Insect
    Promoter
    same pP[RS3]
    Available Since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. pLCHKOv3-intergenic-As

    Plasmid
    #209026
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209026-plasmid-map-sequence-id-413692

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  34. pLCHKOv3-intergenic-Lb

    Plasmid
    #209030
    Purpose
    Lentiviral vector expressing U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & LbCas12a DR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209030-plasmid-map-sequence-id-413690

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  35. mitoTALECD for_-20G_1333CN

    Plasmid
    #186200
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted 20th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Available Since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186200-plasmid-map-sequence-id-368306

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  36. mitoTALECD for_ -13G_1397CN

    Plasmid
    #186199
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -13th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Available Since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186199-plasmid-map-sequence-id-368290

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  37. mitoTALECD for_ -6G_1333CN

    Plasmid
    #186198
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -6th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Available Since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186198-plasmid-map-sequence-id-368288

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Showing: 2241 - 2280 of 15661 results