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pCAG-GluA1-scFV-Halo
Plasmid#230065PurposeExpresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
UseTagsHaloTagExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
RIX-F2Oct4F3-GFP
Plasmid#216848PurposeThis plasmid is for the production of lentiviral vectors that can be used to track pluripotent stem cells, such as ES cells, iPS and tissue-resident pluripotent cells. GFP expression is lost upon diffDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterOct4Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOpen-Endonuclease IV E. coli
Plasmid#165551PurposeDNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.DepositorInsertEndonuclease IV E. coli
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PRDX1A-c001
Plasmid#175473PurposeProtein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.DepositorInsertPRDX1 (PRDX1 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX-HA(NcoI/EcoRI)
Plasmid#15405DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1 and HAExpressionBacterialMutationinsert can be released by digestion with REs NcoI…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBW001
Plasmid#131529PurposeVector containing the Mu-BsaI-KanR transposon that can be excised with HindIII and BglII for transposition reactionsDepositorArticleInsertMu-BsaI-KanR transposon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human Coxsackievirus A16 strain G10 2A protease
Plasmid#228632PurposeBacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)DepositorInsertEnterovirus Coxsackievirus A16 2A protease (HEVAgp1 Coxsackievirus 16)
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGYL1_pPromALB_FireflyLuc
Plasmid#220282PurposeFirefly luciferase with minimal Albumin promoter without enhancer; enhancer of interest can be cloned into MCSDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
Plasmid#226590PurposePharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.DepositorInsertPSAML-141F,Y115F-GlyR
UseAAVTagsP2A-Fusion RedExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp
Plasmid#212144PurposePreparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp_CamRcargo
Plasmid#212143PurposePreparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA
Plasmid#99698PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAVDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GluA1-scFv-Halo
Plasmid#230055PurposeAAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
UseAAVTagsHaloTagExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
GW1CMV-Perceval
Plasmid#21737Purposecan be used to monitor the ATP:ADP ratio during live-cell imagingDepositorInsertPerceval (fluorescent ATP:ADP sensor)
UseTags5'UTRExpressionMammalianMutationimproved Kozak sequencePromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194715PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sitesDepositorArticleInsertAsCpf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Flex-Axon-GCaMP7b
Plasmid#135419PurposeCan be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.DepositorInsertaxon-jGCaMP7b
UseAAVTags6xHis and GAP43 palmitoylation domainExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s
Plasmid#105715PurposeIn the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.DepositorHas ServiceAAV5Insertsynaptophysin-GCaMP6s
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1a, Human elongation factor-1 alphaAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRL488
Plasmid#70263Purposedesigned to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.DepositorTypeEmpty backboneUseLuciferase; Cyanobacteria expressionTagsluxABExpressionMutationPromoternoneAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZE-P9-egfp
Plasmid#201790Purposethe promoter can be recognized by PadRDepositorInsertP9
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only
