We narrowed to 5,093 results for: puc

1. pAAV-CMV-dSa VP64 Afp

   Plasmid

   #99697

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV

   Depositor

   George Church

   Insert

   dCas9 and gRNA targeting Afp (Afp Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Mutation

   dead Cas9

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCM54

   Plasmid

   #220830

   Purpose

   EryA sensor with increased sensitivity due to phosphorylation of EryA by mphA, which traps EryA in cell

   Depositor

   Matthew Bennett

   Article

   Miller et al ACS Synth Biol. 2021 Feb 19;10(2):258-26

   Insert

   mphA
   

   Promoter

   Pmph

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCM98

   Plasmid

   #220831

   Purpose

   EryA sensor with further increased sensitivity by mutation of mphR (T17R), in addition to mphA phosphorylation

   Depositor

   Matthew Bennett

   Article

   Miller et al ACS Synth Biol. 2021 Feb 19;10(2):258-26

   Insert

   mphR
   

   Mutation

   T17R

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKPcR5

   Plasmid

   #149467

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, PcaU(AM) repressor, P3B5B promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   PcaU(AM)-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   P3B5B

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKVR5

   Plasmid

   #149469

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, VanR(AM) repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   VanR(AM)-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PVanCC

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. VGF8a

   Plasmid

   #107572

   Purpose

   partial cDNA of a neurotrophin responsive gene

   Depositor

   Andrea Levi, Bruce Paterson

   Article

   Levi et al Science. 1985 Jul 26;229(4711):393-5.

   Insert

   VGF8a (Vgf Rat)
   

   Use

   Cloning vector

   Mutation

   partial cDNA (amino acid 65 - 617)

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNSEN-d2

   Plasmid

   #59763

   Purpose

   Expresses nuclear EGFP and Tomato in mammalian cells

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi

   Inserts

   d2-EGFP
   Tomato
   

   Tags

   3X NLS and PEST

   Expression

   Mammalian

   Promoter

   CMV promoter and SV40 promoter

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEVA3b61

   Plasmid

   #58310

   Purpose

   Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.

   Depositor

   Tom Ellis

   Article

   Wright et al ACS Synth Biol. 2014 May 13.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM5411

   Plasmid

   #132663

   Purpose

   RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Inserts

   aadA
   YFP
   

   Expression

   Bacterial

   Promoter

   PaadA and PconII

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pREDusk-MCS

    Plasmid

    #188970

    Purpose

    For red light-repressed bacterial expression of gene of interest

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJKR-H-tetR

    Plasmid

    #62561

    Purpose

    Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. KG#908

    Plasmid

    #110914

    Purpose

    Expresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegans

    Depositor

    Kenneth Miller

    Inserts

    rab-3 promoter
    TIR1 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. EZ-L560

    Plasmid

    #173028

    Purpose

    Encodes OptoAMP2 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Available Since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. EZ-L175

    Plasmid

    #173025

    Purpose

    Encodes OptoAMP1 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJKR-H-mphR

    Plasmid

    #62559

    Purpose

    Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJKR-H-araC

    Plasmid

    #62563

    Purpose

    Arabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJKR-H-acuR

    Plasmid

    #62567

    Purpose

    Acrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pED17x11

    Plasmid

    #134814

    Purpose

    sfGFP expression plasmid for characterizing qtRNA charging

    Depositor

    Ahmed Badran

    Article

    DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

    Insert

    sfGFP-151-TAGA
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    151-TAGA

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7sgRNA

    Plasmid

    #111820

    Purpose

    Expresses sgRNA in T. brucei cells

    Depositor

    David Horn

    Article

    Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

    Insert

    sgRNA
    

    Use

    CRISPR

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJKR-H-ttgR

    Plasmid

    #62565

    Purpose

    Flavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge