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pTL-Bag2T-gRNA2

Plasmid

#196253

Purpose

Plasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAs

Depositor

Bing Yang

Publication

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

CRISPR-Cas9 guide RNA scaffold and multiple cloning site

Expression

Bacterial

Promoter

J23119 promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 MERS-CoV NSP3_nostop

Plasmid

#168608

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP3 (orf1ab )

Use

Other

Available since

Jan. 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 HCoV-HKU1 NSP1_nostop

Plasmid

#168570

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP1 (ORF1ab )

Use

Other

Available since

Jan. 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-1 NSP6_stop

Plasmid

#168548

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP6 (ORF1ab )

Use

Other

Available since

Jan. 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-Spe3-RfA

Plasmid

#186370

Purpose

Destination vector for the expression of a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAT079

Plasmid

#180509

Purpose

Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Publication

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv2
DpbCasX

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Promoter

CAG and U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-pegRNA-tetra-com vector

Plasmid

#136271

Purpose

Expresses PE2 and Tetra-com modified pegRNA cassette

Depositor

Baisong Lu

Insert

PE2 and pegRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8_MF-PH-HA-Cter (VE5629)

Plasmid

#139775

Purpose

Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter HA tag under the pH promoter.

Depositor

Arnaud Poterszman

Publication

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

C-terminal Hemaglutinine tag

Tags

HA

Promoter

PH

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-R-Hras

Plasmid

#180579

Purpose

ddRFP sensor of Hras

Depositor

Won Do Heo

Publication

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Hras

Use

AAV

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRVdG-4FLPo

Plasmid

#122050

Purpose

Genome plasmid for deletion-mutant rabies virus encoding Flpo recombinase

Depositor

Ian Wickersham

Publication

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

FLPo

Expression

Mammalian

Promoter

CMV

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBlastR-5xUAS-mCherry-MiniWhite

Plasmid

#165911

Purpose

Blasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable UAS Response Vector

Use

Synthetic Biology

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4R3_T CTP

Plasmid

#171736

Purpose

ptpTALECD vector assembly, Entry clone 2 with attR4 and R3

Depositor

Shin-ichi Arimura

Publication

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal

Use

Multisite gateway entry vector

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUHD-SB-AID/Bla

Plasmid

#171678

Purpose

TRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (blasticidin)

Depositor

Randy Poon

Publication

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Tags

AID

Expression

Mammalian

Promoter

TRE

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pUHD-SB-AID/Hyg

Plasmid

#171677

Purpose

TRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (hygromycin)

Depositor

Randy Poon

Publication

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Tags

AID

Expression

Mammalian

Promoter

TRE

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMS27: pTns(PmcCAST)_ΔTnsD

Plasmid

#168160

Purpose

Inducible expression of PmcCAST TnsAB, TnsC and TniQ proteins.

Depositor

Feng Zhang

Publication

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ

Expression

Bacterial

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS25: pTns(PmcCAST)

Plasmid

#168158

Purpose

Inducible expression of PmcCAST TnsAB, TnsC, TniQ and TnsD proteins.

Depositor

Feng Zhang

Publication

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ

Expression

Bacterial

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS10: pTns(AvCAST)_ΔTnsD

Plasmid

#168143

Purpose

Inducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.

Depositor

Feng Zhang

Publication

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)

Expression

Bacterial

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRMA154

Plasmid

#167554

Purpose

S. flexneri O antigen genes cloned from pPM2213 into pJRD215

Depositor

Renato Morona

Publication

Morona et al J Bacteriol. 1994 Feb;176(3):733-47. doi

Insert

S. flexneri O antigen genes cloned from pPM2213 (ClaI - KpnI)

Use

Broad host range mobilisable

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-12xLexAOp-EBFP2-MiniWhite

Plasmid

#165918

Purpose

Blasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xUAS-sfGFP-MiniWhite

Plasmid

#165910

Purpose

Blasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable UAS Response Vector

Use

Synthetic Biology

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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