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pAG657 H2B-pFAST

Plasmid

#172862

Purpose

Expresses pFAST fused to zebrafish histone H2B in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

H2B-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mCardinal-PMP-N-10

Plasmid

#56173

Purpose

Localization: Peroxisomes, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

PMP (PXMP2 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
TOPO tTR-KRAB-rtTA3-Luc

Plasmid

#68449

Purpose

TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

tTR-KRAB-2A-rtTA3-2A-Luciferase

Use

Luciferase; Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCXLE-hS + eGFP

Plasmid

#74945

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

Sox2 (SOX2 Human)

Expression

Mammalian

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only
mCerulean3-Golgi-7

Plasmid

#55420

Purpose

Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Golgi (B4GALT1 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
mTagRFP-T-Actin-7

Plasmid

#58001

Purpose

Localization: Actin, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Actin (ACTB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCellulose

Plasmid

#36300

Depositor

Jay Keasling

Article

Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

Insert

pCellulose

Use

Synthetic Biology

Promoter

Two promoters from the E coli genome drive two di…

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only

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Dendra2-Vinculin-N-21

Plasmid

#57749

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

NM_003373.3 (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pBV-luc TOP2A 1kb promoter

Plasmid

#138274

Purpose

Luciferase reporter plasmid driven by TOP2A promoter

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TOP2A promoter sequence

Use

Luciferase

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pICH67131

Plasmid

#153223

Purpose

Level 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Nos promoter, NPTII, Nos terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mApple-M1 SpastinK388R

Plasmid

#134463

Purpose

M1 SpastinK388R expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Expression

Mammalian

Mutation

Lys to Arg at amino acid 388

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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LB124 (Bcl-2 from -1287 to -3934)

Plasmid

#15240

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -3934 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
attB-mCh-Poly(A)

Plasmid

#65525

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-mCherry

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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mRuby2-H2B-C-10

Plasmid

#55898

Purpose

Localization: Nucleus/Histones, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

H2B (H2BC11 Human)

Tags

mRuby2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
attB-GFP-Poly(A)‐NeoR

Plasmid

#65526

Purpose

NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6CNW)RS

Plasmid

#222873

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEB2-mCherry-L

Plasmid

#104002

Purpose

Plasmid encoding mCherry-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCherry-L

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGEE DNLA IIKEF… V…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBeast-pAhpC-RBS- LacZ

Plasmid

#190060

Purpose

LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

lacZ

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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AA025

Plasmid

#216005

Purpose

Fragmid fragment: (Cas protein) NG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9-NG_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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BAT-gal

Plasmid

#20889

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7XTCF binding sites, Siamois minimal promoter, NLS-lacz

Use

Mouse Targeting

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements cloned upstre…

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only