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pICSL1P5_crU626

Plasmid

#191780

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II CC

Plasmid

#35180

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains C-terminal domain repeats (1-22)X2

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
BSA2_His

Plasmid

#202767

Purpose

bovine serum albumin design BSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA2

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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BSA3_His

Plasmid

#202768

Purpose

bovine serum albumin design BSA3, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA1

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDD399

Plasmid

#91821

Purpose

AID CT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pJ291_WT_med

Plasmid

#128856

Purpose

Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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475 p5EfliEBSbasP

Plasmid

#26027

Depositor

Nathan Lawson

Article

Villefranc et al Development. 2013 Apr;140(7):1497-506. d

Insert

fli1a intron 1

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcD-10xHis)

Plasmid

#176192

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

10xHis

Expression

Bacterial

Promoter

Ptac

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMCSG26(gfcD)

Plasmid

#176198

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcBCD)

Plasmid

#176193

Purpose

expresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operon

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcBCD

Expression

Bacterial

Promoter

Ptac

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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512 pME-miR

Plasmid

#26032

Depositor

Nathan Lawson

Article

Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

Insert

ef1a (eef1a1l1 Zebrafish)

Use

Entry vector

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only
pLEX_307_ms2_VP64

Plasmid

#79375

Purpose

Recruits VP64 to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VP64

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBT100.110

Plasmid

#122602

Purpose

clone CDA1 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

CDA1

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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C-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45222

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

C-term Hec1-targeted Aurora B FRET sensor

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro4GFP

Plasmid

#82368

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro4GFP

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/N149C/S202C

Promoter

T7

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCMB-PERr

Plasmid

#61203

Purpose

Fluorescent reporter for peroxisomes mCherry-SKL expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

mCherry-SKL

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin2-S190W

Plasmid

#73670

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S190W changed serine 190 to tryptophan

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pLJM1-FLAG-Sestrin2-E451A

Plasmid

#73677

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
258/-2.5lacZ

Plasmid

#25429

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Development. 1995 Mar . 121(3):637-49.

Insert

Myo D Promoter (MYOD1 Human)

Use

Reporter construct

Tags

lacZ

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pDB026g

Plasmid

#109263

Purpose

Accessory plasmid for PACE of MjTyrRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp_pIII(P29*, P83*, T177*, Y184*); luxAB; PproK_TyrT(Opt, CUA);

Expression

Bacterial

Mutation

amber codon at position 29, 83, 177, and 184 of g…

Promoter

Ppsp and PproK

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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