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pRS415-SOH1

Plasmid

#8622

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

SOH1 (SOH1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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SYNJ1_Halo_C_allele

Plasmid

#178148

Purpose

Donor vector for endogenous tagging of human SYNJ1 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (SYNJ1 Human)

Use

Donor vector

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pQE-WT-N4-R5-YRNRV

Plasmid

#63625

Purpose

N4-R5-YRNRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

N4-R5-YRNRV RNAP

Mutation

N671Y, L747I, N748D, L749C, M750V, F751I, Q754T, …

Promoter

T5/Lac

Available Since

June 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pSW104

Plasmid

#122295

Purpose

For run-off transcription of yeast RPL41A mRNA with and without an 82 nucleotide poly(A) tail.

Depositor

Sarah Walker

Article

Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

Insert

RPL41A

Use

In vitro transcription

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9m3

Plasmid

#47315

Purpose

Cas9 D10A+D839A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m3

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
JB88

Plasmid

#128933

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

RhebSN-BirA*-HA-MAO (RHEB Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB87

Plasmid

#128932

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

RhebQL-BirA*-HA-MAO (RHEB Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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YEplac181-TYS1

Plasmid

#8629

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

TYS1 (TYS1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pRubiC-T2A-cre

Plasmid

#66692

Purpose

Recombine floxed alleles in newborn neurons

Depositor

Bryan Luikart

Article

Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

Insert

mCherry-T2A-Cre

Use

Retroviral

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTK715

Plasmid

#114698

Purpose

Express NuMA(1-2115)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-2115)-RFP-Nano

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-FLAG-hG9a

Plasmid

#33023

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

5'LTR

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
C121-E12: NanoLuc

Plasmid

#162891

Purpose

Standard Gateway entry clone encoding NanoLuc luciferase

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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IDP205 (T7->Rci; 5bc)

Plasmid

#68834

Purpose

Expresses Rci gene (driven by the inducible T7 promoter) and 5 fragment barcode cassette in bacterial cells

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2014;42(16):e127. doi

Inserts

Barcode cassette 5 fragments
Rci recombinase

Promoter

T7

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Ube2d2

Plasmid

#31410

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2D2 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pTHSSd_10

Plasmid

#59950

Purpose

PK1F GFP reporter

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

sfGFP

Expression

Bacterial

Mutation

N/A

Promoter

PK1F

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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CHCHD10_Halo_N_allele

Plasmid

#178118

Purpose

Donor vector for endogenous tagging of human CHCHD10 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (CHCHD10 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134155

Plasmid

#152416

Purpose

Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available Since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRRL.gp91.Syn.Intron.eGFP

Plasmid

#30467

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

gp91/Syn.Intron/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGMCZ-PrpoB

Plasmid

#27073

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P-rpoB

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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Slax mMyf5 (CT#1008)

Plasmid

#13913

Depositor

Cliff Tabin

Article

Brent et al Development. 2005 Feb . 132(3):515-28.

Insert

Myf5 (Myf5 Mouse)

Expression

Bacterial

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only