We narrowed to 30,936 results for: LIS;

1. PL-SIN-PGK-EiP

   Plasmid

   #21312

   Purpose

   Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP and Puro resistance

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. PL-SIN-EOS-S(4+)-EiP

   Plasmid

   #21314

   Purpose

   Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EOS-S(4+) promoter region

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUASTattB-dALFANb(5mut)-mGreenLantern

   Plasmid

   #219661

   Purpose

   Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern
   

   Expression

   Insect

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Burden Monitor lambda version

   Plasmid

   #66073

   Purpose

   The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   GFP
   

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pBS4S

   Plasmid

   #55170

   Purpose

   Empty vector, integration at thrC, ampr, specr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   none

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

6. SPDK3860

   Plasmid

   #199245

   Purpose

   Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Promoter

   pPEBV

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

   Plasmid

   #83485

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R472A_H477A_R1048A_H1053A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A, R1048A, H1053A

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. HSC1-GiP

   Plasmid

   #58254

   Purpose

   This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

   Depositor

   James Ellis

   Article

   Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

   Insert

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Human EF1-alpha

   Available Since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115210

   Purpose

   Expresses AspCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   AspCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p3869 HA-E6-AP C833A

    Plasmid

    #8649

    Depositor

    Peter Howley

    Article

    Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

    Insert

    E6-AP (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C833A; active site Cys mutated to encode Ala

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

11. CAG sHRPb-FKBP-post mGRASP

    Plasmid

    #73146

    Purpose

    Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPb-FKBP-post mGRASP
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRevTRE-hygro-GFP-Crumbs3a WT

    Plasmid

    #195217

    Purpose

    Retroviral Tet response vector containing GFP tagged Crumbs3a

    Depositor

    Ben Margolis, David Bryant

    Article

    Schluter et al Mol Biol Cell. 2009 Nov;20(22):4652-63.

    Insert

    CRB3A (CRB3 Human)
    

    Use

    Retroviral

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNAintron-CHIKV-Envelope protein

    Plasmid

    #215700

    Purpose

    Produces the Chikungunya virus envelope protein E3-E2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV E3-E2-6K-E1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pUASTattB-dALFANb(3mut)-mGreenLantern

    Plasmid

    #219662

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern
    

    Expression

    Insect

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-hL1

    Plasmid

    #89411

    Purpose

    mammalian expression of hL1

    Depositor

    Fritz Rathjen

    Article

    De Angelis et al EMBO J. 1999 Sep 1. 18(17):4744-53.

    Insert

    L1CAM (L1CAM Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS1ClacZ

    Plasmid

    #55171

    Purpose

    lacZ-reporter vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

17. Burden Monitor phi80 version

    Plasmid

    #66074

    Purpose

    The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

    Insert

    GFP
    

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac1-Ls-AChBP

    Plasmid

    #63126

    Purpose

    Expression of Lymnaea stagnalis AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(-)-EGFP-FP4-mito

    Plasmid

    #229692

    Purpose

    Transient expression of EGFP FP4-mito in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP; Mitochondira FP4 motifs from Listeria ActA
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Gad65P-eGFP-3XNLS-WPRE-pA

    Plasmid

    #183804

    Purpose

    AAV vector to express nuclear localized eGFP from a Gad65 promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

    Insert

    eGFP-NLS
    

    Use

    AAV

    Tags

    NLS

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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