We narrowed to 24,135 results for: crispr

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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p3s-eCas9-1.1

Plasmid

#104172

Purpose

eCas9-1.1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

eCas9-1.1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-CLIC3

Plasmid

#140583

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA CLIC3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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Cas9m2-VP64

Plasmid

#47317

Purpose

Cas9m2 Activator

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m2-VP64

Use

CRISPR

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pARS3_MUbCAS9_MC

Plasmid

#128767

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS for cloning gRNA

Depositor

Zachary Nimchuk

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Tags

HA

Expression

Plant

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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XX>sgRNA F+E

Plasmid

#87543

Purpose

Template 2 (of 2) for U6>sgRNA cassette OSO-PCR

Depositor

Lionel Christiaen

Article

Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

Type

Empty backbone

Available Since

Jan. 27, 2018

Availability

Academic Institutions and Nonprofits only

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BCJJ358

Plasmid

#117505

Purpose

RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtRPS5a

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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BCJJ344

Plasmid

#117503

Purpose

UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtUBI10

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2

Plasmid

#116953

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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library backbone 3'LTR gRNA and barcode

Plasmid

#127397

Purpose

Digestion by BstXI and BamHI to release the backbone

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

Type

Empty backbone

Use

Lentiviral

Tags

Puro-mCherry

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMLS544

Plasmid

#188882

Purpose

Integrating Pmex-5::Cas9 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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BCJJ340

Plasmid

#117502

Purpose

UBI10:Cas9:E9 Golden Gate Level 1 Position 2

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtUBI10

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-VEGFA

Plasmid

#140588

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA VEGFA

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pU6Rosa-CAG-Cas9

Plasmid

#118998

Purpose

Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Cas9

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2367

Plasmid

#160076

Purpose

Z. mobilis CRISPRi, promoter C, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS546

Plasmid

#188883

Purpose

Integrating Pmex-5::Cas9 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Expression

Worm

Mutation

C. elegans codom optimization

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAS_gpyrG1

Plasmid

#90276

Purpose

(also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

gRNA (pyrg1)

Use

A. niger

Expression

Bacterial

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pOPO640

Plasmid

#195296

Purpose

pTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

miniMcCAST (Tn7575) with KanR

Expression

Bacterial

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9m3-VP64

Plasmid

#47318

Purpose

Cas9m3 Activator

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m3-VP64

Use

CRISPR

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pmCherry-U6-CACNG3

Plasmid

#140590

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA CACNG3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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