We narrowed to 17,223 results for: EMB
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Plasmid#103071PurposeCas9 coding gene template with optimized sequence for human codon usage, also expresses EGFPDepositorInsertB2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
UseCRISPRExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgMAPKAP1_2
Plasmid#124911PurposeTargeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9DepositorInsertMAPKAP1 (MAPKAP1 Human)
UseCRISPR and LentiviralAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgMAPKAP1_3
Plasmid#124913PurposeTargeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9DepositorInsertMAPKAP1 (MAPKAP1 Human)
UseCRISPR and LentiviralAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN1 VAMP2 R125TAG
Plasmid#69877Purposeexpress in mammalian cells for unnatural amino acid incorporation into VAMP2DepositorInsertVAMP2 (Vamp2 Rat)
UseSynthetic BiologyExpressionMammalianMutationAmber and Ochre stop codons in the former linker …Available SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-tet-puro-sh-ex16
Plasmid#35167DepositorAvailable SinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1 + VP2C-GFP
Plasmid#166676PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertsVP2C-GFP
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyExpressionYeastPromoterGAL1 and GAL10Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a C2AB
Plasmid#40037DepositorInserthSlp4-a C2AB (SYTL4 Human)
TagsEGFPExpressionMammalianMutationC2A and C2B domains (353-671)PromoterCMV promoterAvailable SinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGAS R71/75E C396/7A-HA
Plasmid#130920PurposeExpresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection markerDepositorInserthuman cGAS R71/75E C396/7A
TagsHAExpressionMammalianMutationaa 71 and 75 mutated to glutamate; aa 396 and 397…PromoterCMVAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a C2A
Plasmid#40038DepositorInserthSlp4-a C2A (SYTL4 Human)
TagsEGFPExpressionMammalianMutationC2A domain (353-487)PromoterCMV promoterAvailable SinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
nes374tk/lacZ
Plasmid#47615Purposeused to create transgenic mice expressing LacZ reporter with Nestin enhancerDepositorInsertnestin 2nd intron fragment (NES Human)
UseEnhancer reporterTagsHSV tk promoter and lacZMutation374 bp from 2nd intron (comprising bp 1153 to 152…PromoterHSV tk promoterAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 SHPmut
Plasmid#113503PurposeBacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP boxDepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationF215A/E218A/QKL220-222AAAAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a C2B
Plasmid#40039DepositorInserthSlp4-a C2B (SYTL4 Human)
TagsEGFPExpressionMammalianMutationC2B domain (488-671)PromoterCMV promoterAvailable SinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 hSlp2-a del.C2AB
Plasmid#40059DepositorInserthSlp2-a del.C2AB (SYTL2 Human)
TagsGSTExpressionBacterialMutationdeletion of C2A and C2B domains (601-910)PromoterTacAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
mPapaya-Farnesyl-5
Plasmid#56643PurposeLocalization: Membrane, Excitation: 530, Emission: 541DepositorInsertFarnesyl (HRAS Human)
TagsmPapayaExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable SinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp2-a V18A
Plasmid#40054DepositorInserthSlp2-a V18A (SYTL2 Human)
TagsEGFPExpressionMammalianMutationValine 18 to AlaninePromoterCMV promoterAvailable SinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 deltaUBX
Plasmid#113504PurposeBacterial expression of GST-tagged p37 lacking the UBX domainDepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationdelta 251-331 (stop codon behind AA250)Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-tet-puro-sh-ex16b
Plasmid#35168DepositorAvailable SinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 Slp2-a C2AB
Plasmid#40058DepositorInsertSlp2-a C2AB (Sytl2 Mouse)
TagsGSTExpressionBacterialMutationC2AB domains onlyPromoterTacAvailable SinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Slp2-a C2B
Plasmid#40053DepositorInsertSlp2-a C2B (Sytl2 Mouse)
TagsEGFPExpressionMammalianMutationC2B domainPromoterCMV promoterAvailable SinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL
Plasmid#58414PurposeExpresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
TagsHA internalExpressionMammalianMutationCysteines 71, 72, and 105 to Alanine; The sequenc…PromoterCMV IEAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only