159,557 results

1. pBABE-puro-GFP-progerin

   Plasmid

   #17663

   Depositor

   Tom Misteli

   Article

   Scaffidi et al Nat Cell Biol. 2008 Mar 2. ():.

   Insert

   GFP-D50 lamin A (LMNA Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Delta 50

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

   Plasmid

   #162378

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   Synapsin

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. cpLID-micro

   Plasmid

   #168995

   Purpose

   circularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)

   Depositor

   Yubin Zhou

   Article

   He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

   Insert

   mCh-sspB-T2A-cpLID-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hSTING (V155M)

   Plasmid

   #214161

   Purpose

   Transient expression of gain-of-function STING mutant

   Depositor

   Nan Yan

   Article

   Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

   Insert

   STING (V155M) (STING1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V155M

   Promoter

   CMV

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBluescript-CTG-47

   Plasmid

   #99150

   Purpose

   47xCAG repeats in pBluescript vector

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   47xCTG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Galphai2-mTurquoise2

   Plasmid

   #69621

   Purpose

   The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Insert

   Galphai2-mTurquoise2
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. BB3cK_pGAP_23*_pTEF_Cas9

   Plasmid

   #104909

   Purpose

   hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEos3.2-TOMM20-N-10

   Plasmid

   #57483

   Purpose

   Localization: Mitochondrial Membrane, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pDHt/sk-PE

   Plasmid

   #92126

   Purpose

   Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei

   Depositor

   Zhihua Zhou

   Article

   Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

   Insert

   Cas9-eGFP
   

   Use

   CRISPR; Trichoderma reesei expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Ppdc

   Available since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE-zeo largeTcDNA

    Plasmid

    #1779

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. R4pGWB601_UBQ10p-Turbo-YFP-NLS

    Plasmid

    #127368

    Purpose

    Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. nos-Cas9.874Z1

    Plasmid

    #112685

    Purpose

    Express hSpCas9 under nanos promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Use

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    nanos

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDF-Frb-v1

    Plasmid

    #201923

    Purpose

    E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    FrbA
    FrbB
    FrbC
    FrbD
    FrbE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (modified), T7 (modified, moderate), T7 (modif…

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-mEGFP-CaMKIIa (T286A)

    Plasmid

    #127390

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Alanine

    Promoter

    pCAG

    Available since

    Jan. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAC-ANPEP

    Plasmid

    #172418

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Aminopeptidase N (AP-N) (CD antigen CD13) (ANPEP Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. PYL1_nTEVp

    Plasmid

    #119213

    Purpose

    Expresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nTEVp in sufion with PYL1
    

    Use

    Tags

    AU1 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-3xFLAG-NLS-SpCas9-NLS

    Plasmid

    #51307

    Purpose

    Cas9 expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Insert

    3xFLAG-NLS-SpCas9-NLS
    

    Use

    CRISPR; Xenopus tropicalis expression

    Tags

    3x Flag and NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pzntA_LacZ

    Plasmid

    #216325

    Purpose

    PZntA promoter driving expression of lacZ

    Depositor

    Mark Styczynski

    Article

    McNerney et al Sci Adv. 2019 Sep 25;5(9):eaax4473. doi:

    Insert

    b-Galactosidase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLV.5’-LMNA-eGFP-KI

    Plasmid

    #170545

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for LMNA knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP12754 - pAAV-AiE0680m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2754)

    Plasmid

    #224004

    Purpose

    AiE0680m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L2/3_IT neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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