162,325 results

1. pBAD-Padron2

   Plasmid

   #172355

   Purpose

   Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

   Insert

   Padron2
   

   Use

   Tags

   6xHisTag (N terminal on backbone)

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCXIB-H2B-mCherry

   Plasmid

   #164244

   Purpose

   retroviral plasmid for H2B-mCherry

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   H2B
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti PGK GFP Blast (w510-5)

   Plasmid

   #19069

   Purpose

   3rd gen lentiviral eGFP expression vector, PGK promoter, Blast

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-nEF-Con/Fon DREADD Gi-mCherry

   Plasmid

   #177672

   Purpose

   Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells

   Depositor

   Karl Deisseroth

   Has Service

   AAV8

   Insert

   DREADD Gi-mCherry
   

   Use

   AAV, Cre/Lox, and Mouse Targeting ; Intrsect

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

   Plasmid

   #215014

   Purpose

   Express an autophagy (LC3) reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   MAP1LC3B (Map1lc3b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEYFP-N3-EPAC1

   Plasmid

   #113110

   Purpose

   Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

   Depositor

   Xiaodong Cheng

   Article

   Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

   Insert

   RapGEF3 (RAPGEF3 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   PCMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-3xFLAG-Blast

   Plasmid

   #207717

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. S48I48 ELP

   Plasmid

   #68394

   Purpose

   Elastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   S48I48 ELP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2049

   Plasmid

   #143866

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PURA (PURA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICSL11017 (pICH47732::NOSp-BAR-NOST)

    Plasmid

    #51145

    Purpose

    Confers Basta resistance in planta

    Depositor

    Jonathan D Jones

    Insert

    NOSp-BAR-NOST
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Desmoglein2-mCherry

    Plasmid

    #36991

    Depositor

    Kathleen Green

    Article

    Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

    Insert

    Dsg2 (DSG2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. ColorfulCell

    Plasmid

    #62449

    Purpose

    MXS_chaining vector with 6 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMV::GTS-Citrine-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::NLS-TagBFPx3-bGHpA
    CMV::MTS-AzamiGreen-bGHpA
    CMV::iRFP670-SKL-bGHpA
    CMV::ETS-mCherry-KDEL-bGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET16b-Trx

    Plasmid

    #36944

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. ATP5F1B-pmCherry

    Plasmid

    #232783

    Purpose

    Encodes an mCherry-tagged ATP5F1B

    Depositor

    Giulia Bertolin

    Article

    Sharma et al Cell Death Discov. 2023 Jun 29;9(1):203.

    Insert

    ATP5F1B (ATP5F1B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMV1 pEF1a-HGF

    Plasmid

    #188748

    Purpose

    Vector constitutively expressing HGF

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    HGF (HGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pShHELIX_sgRNA_entry (CJT32)

    Plasmid

    #181782

    Purpose

    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLDLR-Luc (aka: pES7)

    Plasmid

    #14940

    Depositor

    Axel Nohturfft

    Article

    Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    LDLR promoter (LDLR Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. FRB-NLuc

    Plasmid

    #31181

    Depositor

    David Piwnica-Worms

    Article

    Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-puro U6 sgRNA BfuAI stuffer

    Plasmid

    #50920

    Purpose

    U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. CXCR4-Tango

    Plasmid

    #66262

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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