171,035 results

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pET30b-5M SrtA with TEV site

Plasmid

#86962

Purpose

Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

Depositor

Ron Bose

Article

Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

Insert

S.aureus SrtA 5M

Tags

6x Histidine tag and TEV site

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd

Plasmid

#80950

Purpose

Expresses Gsdmd in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBR HPV31 9E

Plasmid

#221009

Purpose

Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322

Depositor

Alison McBride

Insert

Human papillomavirus type 31 genome 9E isolate

Use

Cloning vector

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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TARDBP_Halo_N_allele

Plasmid

#178131

Purpose

Donor vector for endogenous tagging of human TARDBP at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (TARDBP Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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tdTomato-KIF1A

Plasmid

#102850

Purpose

Overexpresses KIF1A and cell fill TdTomato

Depositor

Erik Dent

Article

McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

Insert

KIF1A (Kif1a Rat)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-ChR2-mCherry

Plasmid

#137142

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS

Plasmid

#238243

Purpose

For integration of mCherry_P2AT2A_GFP-nb-2xKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-2xKS

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-ATF6

Plasmid

#32955

Purpose

For visualization of GFP-ATF6 in mammalian cells.

Depositor

Ron Prywes

Article

Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

Insert

ATF6 (ATF6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

M67L and M313I

Promoter

CMV

Available Since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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PS Intein

Plasmid

#242019

Purpose

Blue-light-induced reconstitution of split mCherry.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

PS Intein

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRCC-K

Plasmid

#81191

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-AncBE4stem

Plasmid

#208766

Purpose

AncBE4max that co-expresses UGI and p53DD

Depositor

Hyuk-Jin Cha

Article

Park et al Nat Commun. 2024 May 11;15(1):4002. doi:

Insert

AncBE4max with 2A-UGI and 2A-p53DD

Expression

Mammalian

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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Green cGull

Plasmid

#86867

Purpose

Green fluorescent probe for cGMP

Depositor

Tetsuya Kitaguchi

Article

Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

Insert

Green cGull

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-smURFP

Plasmid

#80349

Purpose

Lentiviral transfer vector under the CMV promoter that expresses smURFP.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Insert

smURFP

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pICH47732

Plasmid

#48000

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-H2B-iRFP720

Plasmid

#128961

Purpose

Lentiviral plasmid for expression of far red iRFP720 nuclear marker

Depositor

Carlos Carmona-Fontaine

Insert

Human histone H2B (H2BC21 Human)

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Promoter

hPGK

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pQE-WT-RGVG-M6

Plasmid

#70172

Purpose

Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

Depositor

Andrew Ellington

Article

Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

Insert

RGVG-M6 T7 RNA polymerase

Tags

His

Expression

Bacterial

Promoter

T5/Lac

Available Since

Dec. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Tet-off TauRD (P301L/V337M)

Plasmid

#188572

Purpose

Dox-repressible expression of the Tau repeat domain

Depositor

Franz-Ulrich Hartl

Article

Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

Insert

Tau repeat domain (MAPT Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

P301L and V337M

Promoter

pTight TRE

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGFPmAID

Plasmid

#174213

Purpose

GFP::mAID donor backbone

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Type

Empty backbone

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAP WT

Plasmid

#123097

Purpose

Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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