165,775 results

1. pcDNA3.1 acGFP

   Plasmid

   #128047

   Purpose

   Mammalian expression vector with N terminal AcGFP

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Tags

   AcGFP

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-2H12.D11

   Plasmid

   #221145

   Purpose

   His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 457 p3E-2A-EGFPpA

   Plasmid

   #195967

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-EGFP
   

   Use

   Other

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR221-BNT162b2ΔUTR

   Plasmid

   #171214

   Purpose

   Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.

   Depositor

   Liang Wei Wang

   Article

   Low et al medRxiv 2021.04.27.21256151

   Insert

   BNT162b2ΔUTR
   

   Use

   Gateway cloning

   Mutation

   Kozak sequence changed from CCCGCCACC to GGCTGCAC…

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.hSynap.iAChSnFR

   Plasmid

   #137950

   Purpose

   Expresses iAChSnFR under hSynapsin promoter

   Depositor

   Loren Looger

   Article

   Borden et al bioRxiv 2020

   Has Service

   AAV1

   Insert

   iAChSnFR
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSynanpsin

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221_SLC6A15

   Plasmid

   #131877

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC6A15 (SLC6A15 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pHel3

   Plasmid

   #102961

   Purpose

   E.coli/H.Pylori shuttle vector

   Depositor

   Rainer Haas

   Article

   Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

   Type

   Empty backbone

   Use

   Shuttle vector

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCitrine-Farnesyl-5

   Plasmid

   #56558

   Purpose

   Localization: Membrane, Excitation: 516, Emission: 529

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available Since

   Dec. 18, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. p663-UBC-miniTurbo-V5-MARK4_IDG-K

   Plasmid

   #189884

   Purpose

   Gateway destination clone of MARK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   MARK4 (MARK4 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Myristoylated optoDroplet

    Plasmid

    #111507

    Purpose

    For membrane localized optoDroplets

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    Cry2 (CRY2 Mustard Weed)
    

    Use

    Lentiviral

    Tags

    FUS, Fusion Red, and Myristoylation Sequence (MYR…

    Promoter

    SFFV

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-HA-DUB3

    Plasmid

    #22593

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    DUB3 (USP17L9P Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL20_mEGFP_PAX3_FOXO1

    Plasmid

    #205875

    Purpose

    Express mEGFP-tagged fusion protein, PAX3_FOXO1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    PAX3_FOXO1 (PAX3, FOXO1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIP-AMPKa1_KD

    Plasmid

    #79011

    Purpose

    For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Expression

    Mammalian

    Mutation

    K47R

    Promoter

    pCAGGs

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK GFP Paxillin

    Plasmid

    #50529

    Purpose

    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Paxillin (PXN Human)
    

    Tags

    GFP21

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20942

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    EF1alpha

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. TMED4-bio-His

    Plasmid

    #51854

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED4 (TMED4 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-PGK-DIO-mCherry-nega miR

    Plasmid

    #137732

    Purpose

    Lentivirus vector for control miR in a Cre-dependent manner

    Depositor

    Tomoyuki Furuyashiki

    Article

    Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

    Insert

    control microRNA
    

    Use

    Lentiviral

    Tags

    mChherry

    Expression

    Mammalian

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRIP-CMV-GFP-FLAG-cGAS

    Plasmid

    #86675

    Purpose

    Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS
    

    Use

    Lentiviral

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMVTNT-T7-KPNA1

    Plasmid

    #26677

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 1 (KPNA1 Human)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    KPNA1 sequence contains a reported SNP at S73N

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid

    #165450

    Purpose

    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6 and short human rhodopsin promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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