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3XFlag pCMV5-TOPO TAZ ∂CC

Plasmid

#24816

Depositor

Jeff Wrana

Publication

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of amino acids 222-265 (coiled coil doma…

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles
pCS2-mkgDUX4

Plasmid

#21156

Depositor

Stephen Tapscott

Publication

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Ub[9]-mRFP[1]

Plasmid

#20249

Depositor

Nico Dantuma

Publication

Salomons et al Mol Cell Biol. 2009 Apr;29(7):1774-85. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ubiquitin C (UBC Human)

Tags

mRFP

Expression

Mammalian

Mutation

changed Stop codon 686 to Glycine

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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TRPML3-YFP

Plasmid

#18835

Depositor

Craig Montell

Publication

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRPML3 (Mcoln3 Mouse)

Tags

YFP

Expression

Mammalian

Mutation

Q165R, F210Y

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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MARCKS(1-40)-paGFP

Plasmid

#18695

Depositor

Karel Svoboda

Publication

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MARCKS(1-40) (MARCKS Human)

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation in paGFP

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-SENP6 (catalytic domain)

Plasmid

#16359

Depositor

Guy Salvesen

Publication

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP6 catalytic domain (SENP6 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 628 to 1112 catalytic domain of human …

Available since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcmvtag2Bmciap2

Plasmid

#11463

Depositor

Jon Ashwell

Publication

Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mciap2 (Birc3 Mouse)

Tags

flag

Expression

Mammalian

Available since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
PKCalpha.K/N.FLAG

Plasmid

#10806

Depositor

Alex Toker

Publication

Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKC alpha (PRKCA Bovine)

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K368N (Lys 368 to Asn)

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pET32a-hEGF

Plasmid

#199231

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Publication

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epidermal growth factor, partial (EGF Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-sfGFP

Plasmid

#199248

Purpose

Fluorescent labelling of bacterial cells with sfGFP

Depositor

Uwe Mamat

Publication

Mamat et al Appl Environ Microbiol. 2023 May 17:e003

Insert

Codon-optimized sfGFP gene

Expression

Bacterial

Mutation

Codon optimization for Xanthomonadaceae

Promoter

Pc promoter from class III integron of Delftia a…

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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XRE-H2B-mScarlet-I

Plasmid

#182296

Purpose

Fluorescent reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Publication

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet-I

Tags

m-Scarlet-I

Expression

Mammalian

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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HLA_A0101

Plasmid

#165009

Purpose

Expression of HLA-A*01:01 cDNA

Depositor

Matthew Meyerson

Publication

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-A*01:01 (HLA-A Human)

Use

Lentiviral

Expression

Mammalian

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSCAR_sgRNA_hygro-tagBFP-lox5171

Plasmid

#162070

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Publication

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ORF3a-EGFP

Plasmid

#165121

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Publication

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ORF3a (ORF3a codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCW-eGFP

Plasmid

#162823

Purpose

Doxycycline-inducible overexpression of eGFP

Depositor

Matthew Steinhauser

Publication

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Mutation

V163A

Promoter

Tet ON

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pet16b-Avi-ubiquitin_rbs_BirA

Plasmid

#134897

Purpose

Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

Depositor

Andrew Deans

Publication

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Inserts

Ubiquitin (UBB Human)
BirA

Tags

10HIS-AviTag-PreScission(3C)protease

Expression

Bacterial

Promoter

RBS and T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-His-TEV-NQO1

Plasmid

#119058

Purpose

For expression in E.coli and affinity purification of N-terminally His tagged NQO1

Depositor

Yosef Shaul

Publication

Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

6His, TEV site

Expression

Bacterial

Promoter

T7

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Mito-DsRed1

Plasmid

#87379

Purpose

Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).

Depositor

Björn Stork

Publication

Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

Insert

TXN2 mitochondrial transit peptide (TXN2 Human)

Use

Retroviral

Tags

DsRed1

Expression

Mammalian

Mutation

First 60 aa only

Promoter

Retroviral 5' LTR promoter

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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VEGFR2-YFP

Plasmid

#108853

Purpose

Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Publication

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KDR (KDR Human)

Tags

Labeled with eYFP on the C-Terminus via a 3 amino…

Expression

Mammalian

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-mRuby3-WPRE

Plasmid

#99279

Purpose

Can be used to generate AAV virus that will express mRuby3 in the presence of Cre

Depositor

Rylan Larsen

Insert

mRuby3

Use

AAV and Cre/Lox

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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