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J23112-mRFP1-331Bb

Plasmid

#78278

Purpose

mRFP1 behind constitutive promoter J23112 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23112-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23103-mRFP1-331Bb

Plasmid

#78273

Purpose

mRFP1 behind constitutive promoter J23103 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23103-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23105-mRFP1-331Bb

Plasmid

#78275

Purpose

mRFP1 behind constitutive promoter J23105 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23105-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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J23101-mRFP1-331Bb

Plasmid

#78272

Purpose

mRFP1 behind constitutive promoter J23101 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23101-mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pAN-PA3-RFP

Plasmid

#62249

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pA3

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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Tmem194 pmRFP-N2 (1389)

Plasmid

#62048

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem194 (TMEM194A Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-RFP-UCR1C

Plasmid

#67582

Purpose

Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Use

Tags

FLAG and TagRFP

Expression

Mammalian

Mutation

Amino acid residues 80-136 of PDE4D3

Promoter

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
pBT2-4xUAS:P2A-mRFP

Plasmid

#127593

Purpose

This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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YCe1731 HC_Kan_RFP-p24

Plasmid

#100634

Purpose

EMMA toolkit part-entry vector position 24

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PLL7.0 tgRFP LOV

Plasmid

#114463

Purpose

Mammalian expression of tgRFP labelled LOV2

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

LOV2

Use

Tags

tgRFP

Expression

Mammalian

Mutation

G401A(G528A), N411E(N538E) and V289I (V416I)

Promoter

CMV

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mRFP-Syx2

Plasmid

#35704

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx2 (Plekhg5 Mouse)

Use

Tags

mRFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pmiRFP670-PCNAL2 (pc3385)

Plasmid

#213140

Purpose

Expresses human PCNA miRFP670 tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Rausch et al Nucleic Acids Res. 2021 Dec 16;49(22):12

Insert

PCNA (PCNA Human)

Use

Tags

miRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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VWF-pHluorin-mRFP

Plasmid

#234367

Purpose

Von Willebrand factor fused in tandem to superecliptic pHluorin and inserted into pmRFP1-N1 vector from Addgene (Plasmid No: 54635)/Monitoring of WPB pH, Visualization of WPB exocytosis

Depositor

Volker Gerke

Article

Terglane et al PLoS One. 2022 Jun 29;17(6):e0270299. do

Insert

Von Willebrand factor (VWF Human)

Use

Tags

pHluorin

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pNX24-RFP-MS2SL

Plasmid

#227819

Purpose

Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

3xHA-RFP-24xMS2SL
Neo resistance

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and SV40

Available since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMaSat-miRFP670 pc3944

Plasmid

#201695

Purpose

Expresses of miRFP-tagged MaSat in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

MaSat Synthetic construct zinc finger

Use

Tags

miRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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tdiRFP-BFP reporter

Plasmid

#190184

Purpose

The cre-activatable fluorescence reporter

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

tdiRFP
TagBFP2

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF-RFP amber

Plasmid

#229524

Purpose

mCherry red fluorescent protein (RFP) reporter protein with an amber stop codon at position 13

Depositor

Thomas Huber

Article

Abdelkader et al Chem 102385

Insert

RFP amber

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-30nmEML-mRFP

Plasmid

#231182

Purpose

Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 30 nm from a lentiviral vector

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-20nmEML-mRFP

Plasmid

#231178

Purpose

Stabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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