We narrowed to 14,014 results for: TIM

1. pNB_EA_R3tdTomatoi2

   Plasmid

   #185969

   Purpose

   Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick
   

   Use

   Synthetic Biology

   Promoter

   PR

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-D1VXP4

   Plasmid

   #103093

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   D1VXP4(Cas9 coding gene from Prevotella timonensis CRIS 5C-B1)
   

   Use

   CRISPR; Cloning vector

   Mutation

   human codon-optimized

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. HeFm1SpCas9

   Plasmid

   #92110

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-GshF-blasticidin

   Plasmid

   #210313

   Purpose

   Expresses humanized Streptococcus thermophilus GshF in mammalian cells

   Depositor

   Kivanc Birsoy

   Article

   Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

   Insert

   Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
   

   Use

   Retroviral

   Tags

   FLAG peptide

   Mutation

   Codon optimized for expression in human cells

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-FNLS-P2A-Puro

   Plasmid

   #110841

   Purpose

   Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTEF:ATP

   Plasmid

   #92179

   Purpose

   HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
   

   Use

   Integration and expression into yeast.

   Tags

   Yeast codon optimized mseCFP (donor) and cp173-mV…

   Expression

   Yeast

   Promoter

   TEF1p

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-TRE3G-FNLS-PGK-Puro

   Plasmid

   #110847

   Purpose

   Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   TRE3G

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mouse b3 full length

   Plasmid

   #217814

   Purpose

   Full length mouse integrin b3 expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGB3 (Itgb3 Mouse)
   

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mouse b8 full length

   Plasmid

   #217817

   Purpose

   Full length mouse integrin b8 expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGB8 (Itgb8 Mouse)
   

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pclbw-opa1(isoform 1)-myc

    Plasmid

    #62845

    Purpose

    Mammalian Expression of myc-tagged Opa1 isoform1

    Depositor

    David Chan

    Article

    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

    Insert

    mmOPA1isoform1-myc (Opa1 Mouse)
    

    Use

    Retroviral

    Tags

    9xMyc

    Expression

    Mammalian

    Promoter

    cmv-bactin

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. (564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9

    Plasmid

    #163025

    Purpose

    Expression of CRISPRi with gRNA against mouse Pcsk9

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9 (Pcsk9 S. aureus)
    

    Use

    AAV

    Tags

    KRAB, SV40 pA, and myc NLS

    Expression

    Mammalian

    Promoter

    Alb-AAT

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MT-AvrXa10-FT

    Plasmid

    #234373

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

    Depositor

    Michael Smanski

    Article

    Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

    Inserts

    NbGP41-GFP-AvrXa10-GB1
    dCas9-24XGP41
    gRNAs targeting FT locus
    RFP
    

    Use

    Synthetic Biology

    Tags

    GB1, GFP, and GP41

    Expression

    Plant

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-BE4GamRA-P2A-Puro

    Plasmid

    #112673

    Purpose

    Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4GamRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-2X-P2A-Puro

    Plasmid

    #110840

    Purpose

    Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-2X
    

    Use

    Lentiviral

    Mutation

    D10A and 2 tandem NLS sequences in the XTEN linker

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-xCas9RA-P2A-Puro

    Plasmid

    #110871

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-FNLS-PGK-Puro

    Plasmid

    #110844

    Purpose

    Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-FNLS
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D10A and NLS sequence at the N-terminus

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-BE4RA-P2A-Puro

    Plasmid

    #112672

    Purpose

    Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mouse a5 full length

    Plasmid

    #217810

    Purpose

    Full length mouse integrin a5 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGA5 (Itga5 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-BE3RA-P2A-GFP-PGK-Puro

    Plasmid

    #110868

    Purpose

    Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-HF1RA-PGK-Puro

    Plasmid

    #110860

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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