We narrowed to 7,235 results for: ALP

1. pCIG3 N

   Plasmid

   #132941

   Purpose

   MLV packaging plasmid for N-tropic MLV gag-pol expression

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   N-tropic MLV gag-pol
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YFP-DAT

   Plasmid

   #90228

   Purpose

   Expresses human DAT in mammalian cells

   Depositor

   Alexander Sorkin

   Article

   Sorkina et al J Biol Chem. 2003 Jul 25;278(30):28274-8

   Insert

   DAT (dopamine transporter) (SLC6A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-surfaceHA-IRES-Puro

   Plasmid

   #207321

   Purpose

   Lentiviral expression of synthetic HA surface receptor in Mammalian cells

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   HAtag-Myctag-PDGFRB-TM-IRES-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha_short (EFS)

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTubulin-miRFP704nano

   Plasmid

   #206192

   Purpose

   Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP704nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Protein Sci. 2023 Jun 22:e4709. doi: 10.

   Insert

   miRFP704nano-human alpha-tubulin
   

   Use

   Tags

   miRFP704nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV3flag8SHARPIN

   Plasmid

   #50014

   Purpose

   Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

   Depositor

   Martin Dorf

   Article

   Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

   Insert

   SHANK-associated RH domain interactor (SHARPIN Human)
   

   Use

   Tags

   3 x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP Tubulin K40R

   Plasmid

   #105303

   Purpose

   To study effects of acetylation-null tubulin

   Depositor

   Kenneth Yamada

   Article

   Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

   Insert

   alpha tubulin (TUBA1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K40R

   Promoter

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUAS-syb:spGFP1–10

   Plasmid

   #73698

   Purpose

   Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP

   Depositor

   Marco Gallio

   Article

   Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

   Insert

   Neuronal-synaptobrevin:spGFP1–10 chimera
   

   Use

   Drosophila

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-6P-1 Stu2 1-590

   Plasmid

   #38314

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCIG3 B

   Plasmid

   #132942

   Purpose

   MLV packaging plasmid for B-tropic MLV gag-pol expression

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   B-tropic MLV gag-pol
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVET-tTR-KRAB

    Plasmid

    #11644

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hEF-1alpha, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FR_HNF6

    Plasmid

    #31099

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF6 (ONECUT1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. KG#62

    Plasmid

    #110874

    Purpose

    Expresses the C. elegans acy-1 cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

    Inserts

    rab-3 promoter
    acy-1 cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLCE-P2A-Puro

    Plasmid

    #123318

    Purpose

    Lentiviral vector for expression of eGFP-P2A-Puro cassette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmCherry-C1-FAK-HA

    Plasmid

    #35039

    Depositor

    Anna Huttenlocher

    Article

    Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

    Insert

    focal adhesion kinase (Ptk2 Mouse)
    

    Use

    Tags

    2x HA and mCherry

    Expression

    Mammalian

    Mutation

    Y42H; deletion of amino acids 1051 & 1052

    Promoter

    Available since

    March 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSIL-eGFP

    Plasmid

    #52675

    Purpose

    shRNA silencing plasmid with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Type

    Empty backbone

    Use

    RNAi

    Tags

    CMV-EGFP

    Expression

    Mutation

    Promoter

    CMV and U6

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pECFP-histone H3

    Plasmid

    #179698

    Purpose

    visualization of nucleus/chromosome

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

    Insert

    Histone H3 (Hist1h3h Mouse)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ND4 DdCBE G1

    Plasmid

    #221978

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ND4 DdCBE C2

    Plasmid

    #221979

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ND4 DdCBE T1

    Plasmid

    #221980

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ND4 DdCBE T2

    Plasmid

    #221981

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EGFP-PIK3R1

    Plasmid

    #174191

    Purpose

    Expresses PIK3R1 in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. psd44-G12QL

    Plasmid

    #46825

    Purpose

    expression of mutant G protein alpha 12 in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    GNA12 (GNA12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Q231L (the mutation reduces GTPase activity resul…

    Promoter

    PGK

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Stat2 pBluescript SK (-)

    Plasmid

    #8705

    Depositor

    Jim Darnell

    Article

    Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

    Insert

    Stat2 (STAT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNL(CMV)Fluc/CMV/WPREdU3

    Plasmid

    #136881

    Purpose

    Reporter vector expression firefly luciferase

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCD/NL-BH∆1

    Plasmid

    #41791

    Purpose

    Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    HIV-1 Gag/Pol and RRE sequences
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCG-Hc∆18-AA-IL-13

    Plasmid

    #86171

    Purpose

    Plasmid encodes blinded measles virus H glycoprotein displaying IL-13

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Measles virus H glycoprotein fused to IL-13
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    Y463A, R515A

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP Tubulin K40A

    Plasmid

    #105304

    Purpose

    To study effects of acetylation-null tubulin

    Depositor

    Kenneth Yamada

    Article

    Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

    Insert

    alpha tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K40A

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRGD-TcR

    Plasmid

    #74110

    Purpose

    Plasmid containing tetracycline resistance gene cassette flanked by multiple restriction sites

    Depositor

    Hans-Martin Fischer

    Article

    Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

    Insert

    tetA(C)
    

    Use

    Bacterial vector based on pmb1 replicon

    Tags

    Expression

    Mutation

    C-to-T transition at pos. no. 1049 of tetA(C) lea…

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hTRAF2pLPCX-HA-Flag/P874

    Plasmid

    #20229

    Depositor

    Martin Dorf

    Article

    Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

    Insert

    TNF receptor-associated factor 2 (TRAF2 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    unmutated NM_021138.3. L replaces M at beginning…

    Promoter

    Available since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBRα

    Plasmid

    #53731

    Purpose

    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    αNTD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CMV-mCh-kCAAX

    Plasmid

    #112267

    Purpose

    ITRACK Acceptor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    mCherry with KRas membrane targeting domain
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TfRΔEctoR1-eGFP A206K

    Plasmid

    #113554

    Purpose

    Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

    Depositor

    Jeanne Stachowiak

    Article

    DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

    Insert

    TfRΔEctoR1-eGFP A206K
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    eGFP A206K

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mCherry-PIK3R1

    Plasmid

    #174192

    Purpose

    Expresses PIK3R1 in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CMV-2mCh-PS

    Plasmid

    #112268

    Purpose

    IDOCKS Acceptor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    two copies of mCherry followed by a pseudosubstrate peptide for PKC
    

    Use

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. BFP-Rab5CA (Q79L)

    Plasmid

    #205705

    Purpose

    Mammalian expression of BFP-tagged mutant Rab5CA

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHRSin_hTCRα

    Plasmid

    #187360

    Purpose

    Lentiviral expression of GPa3b17 hTCR alpha

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    GPa3b17 hTCR alpha
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Human p150,95

    Plasmid

    #8633

    Depositor

    Timothy Springer

    Article

    Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

    Insert

    Human p150,95 (ITGAX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX-6P-1 Stu2 1-306

    Plasmid

    #38315

    Depositor

    Anthony Hyman

    Article

    Widlund et al Mol Biol Cell. 2012 Sep 19.

    Insert

    5' 918 bp from STU2 ORF (STU2 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pNL(CMV)EGFP/CMV/WPRE∆U3

    Plasmid

    #41790

    Purpose

    Lentiviral vector for CMV promoter driven expression of EGFP

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p3A9cbetaTCR

    Plasmid

    #69600

    Purpose

    TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only