We narrowed to 3,025 results for: ER

1. pEF-pIgH-ESCargo(FTV,Orange)

   Plasmid

   #140161

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. roGFP_iE_C147S_pQE30

   Plasmid

   #48631

   Purpose

   Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   roGFP1
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   insertion E147B, C147S

   Promoter

   Available since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSM_ser-2prom3p::myr-mCherry

   Plasmid

   #164223

   Purpose

   Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

   Depositor

   Yan Zou

   Article

   Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

   Insert

   myr-mCherry
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   ser-2promo3

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCI pHluorin Syt5

   Plasmid

   #184506

   Purpose

   Mammalian expression of Synaptotagmin 5 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 5 (Syt5 Mouse)
   

   Use

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMPMA3dPlac-PfliC-GFPLVA

   Plasmid

   #23342

   Depositor

   Olivia Steele-Mortimer

   Article

   Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

   Insert

   GFP(LVA)
   

   Use

   Tags

   GFP(LVA)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSb_init containing the TM287/288 nanobody Nb_TM#2

   Plasmid

   #132698

   Purpose

   expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   nanobody Nb_TM#2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAT024

   Plasmid

   #171636

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BFP
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mito-yHS1

   Plasmid

   #159153

   Purpose

   Mitochondrial labile heme reporter

   Depositor

   Amit Reddi

   Article

   Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

   Insert

   mito-yHS1
   

   Use

   Tags

   COX4 pre-sequence

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pEF NS34a-msGFP-A'Q-mito

   Plasmid

   #158763

   Purpose

   Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Flexible linkers between the protease and msGFP a…

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-4T Syt1 C2AB(140) WT

    Plasmid

    #134695

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purification

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEMT-Map7-1333-end

    Plasmid

    #46070

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-1333-end (Map7 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 RL3Sc

    Plasmid

    #109365

    Purpose

    Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET30_Halotag_K73T_v2

    Plasmid

    #183692

    Purpose

    Bacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    ER Halo(K73T)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K73T in Halotag

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET SUMO-FL-syt1

    Plasmid

    #213628

    Purpose

    Bacterial expression of SUMO-tagged rat FL-syt1

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    FL-syt1 (Syt1 Rat)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMPMA3dPlac-PsopB-GFPLVA

    Plasmid

    #23341

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Use

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SP_H6_Halo_K73T_KDEL_pBABEpu

    Plasmid

    #183689

    Purpose

    Retroviral expression of ER-targeted metastable Halotag/folding sensor (HaloK73T)

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    ER Halo(K73T)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    K73T in Halotag

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNEXdelta-ApAF

    Plasmid

    #13334

    Depositor

    Bong-Kiun Kaang

    Article

    Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

    Insert

    Aplysia activating factor
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pEF NS34a-msGFP-'Q-mito

    Plasmid

    #158764

    Purpose

    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV-2xFLAG-Atoh1ER-DsRed

    Plasmid

    #101735

    Purpose

    Expresses mouse Atoh1 fused to ER and DsRed

    Depositor

    Albert Edge

    Article

    Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2x FLAG, DsRed, and ER

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pECFP-N2-COL1A1

    Plasmid

    #66603

    Purpose

    Expresses procollagen 1 alpha 1 as a CFP fusion

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

    Insert

    COL1A1 (COL1A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only