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Plasmid#82721PurposeExpresses human CREBDepositorInsertCREB (CREB1 Human)
UseTags3XFLAG and GSTExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-controlmRFP1
Plasmid#63848PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromoterJ23100Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CREB
Plasmid#68550PurposeAAV expression of CREBDepositorInsertCREB
UseAAVTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)
Plasmid#224500PurposeGateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)DepositorInsertActin-Vhh-mNeonGreen-HA-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-EGFP-P2A-iCre (JDW 921)
Plasmid#224519PurposeA Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)DepositorInsertEGFP-P2A-iCre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)
Plasmid#229828PurposeA Piggybac compatible, cre dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)
Plasmid#224536PurposeA Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3DepositorInsertIRES-FLAG- NLS-mScarlet-I3
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-mTurquoise-P2A-iCre (JDW 920)
Plasmid#224518PurposeA Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)DepositorInsertmTurquoise-P2A-iCre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP
Plasmid#165090Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pME-mScarlet-I-P2A-iCre (JDW 917)
Plasmid#224516PurposeA Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)DepositorInsertmScarlet-I-P2A-iCre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-lox-3xSTOP-lox (JDW 869)
Plasmid#224521PurposeA Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassetteDepositorInsertLoxP-3xStop-LoxP
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact-EGFP-3xHA (JDW 1322)
Plasmid#224494PurposeGateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)DepositorInsertLiefact-EGFP-3xHA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-mCREB
Plasmid#68551PurposeAAV expression of mCREBDepositorInsertmCREB
UseAAVTagsEGFPExpressionMammalianMutationS133APromoterCMVAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Spike-E
Plasmid#165096Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pME-3xNLS-(G2SG2)2-Cre (JDW 1149)
Plasmid#224514PurposeA Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to CreDepositorInsert3xNLS-(G2SG2)2-Cre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-TetO8x-CMVmin (JDW 1087)
Plasmid#224475PurposeGateway compatible 5' entry clone containing 8 tet operons (TREs) upstream of a minimal CMV promoterDepositorInsertTetO(8x)-CMV promoter
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.2
Plasmid#190796PurposegypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.DepositorInsertgypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest-636
Plasmid#159574PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneUseTagsHis6-MBPExpressionInsectMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) 5xGRE-NanoLuc
Plasmid#207170PurposeGAl4 response element driven NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
5x GRE
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMutationPromoterGal4 response element minP and PGKAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ANKRD24-TwinStrepII
Plasmid#83190PurposeExpression of tagged protein in mammalian cellsDepositorInsertANKRD24 (Ankrd24 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only