We narrowed to 2,064 results for: IPP

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pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

Plasmid

#172984

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

Plasmid

#99229

Purpose

Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos2

Tags

Lifeact

Expression

Mammalian

Mutation

A69T for primed conversion applications

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-Actin-C-18

Plasmid

#53978

Purpose

Localization: Actin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Actin (ACTB Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Dendra2-Vinculin-N-21

Plasmid

#57749

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

NM_003373.3 (VCL Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3-FLAG-LplA(AAG)

Plasmid

#61307

Purpose

Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression

Depositor

Alice Ting

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

Insert

E. coli lipoic acid ligase

Tags

6xHis, Flag, T7 tag, and Xpress epitope

Expression

Mammalian

Mutation

E20A, F147A, H149G

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mKikGR1 (mKikGR1-V69T)

Plasmid

#99227

Purpose

Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mKikGR1

Expression

Bacterial

Mutation

monomeric variant with mutation V69T (Eos notatio…

Promoter

T7

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-S163/164A

Plasmid

#33089

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

S163A, S164A

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

Plasmid

#140228

Purpose

RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

dLbCpf1 (Cas12a) N terminal half (406) with zipper

Use

Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

RRM2 endogenous promoter

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRF+275Dux4

Plasmid

#21293

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX 4 (DUX4 Human, DUX 4)

Use

Luciferase

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pTRE Tight ArchT-GFP

Plasmid

#79627

Purpose

Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

Depositor

Yasunori Hayashi

Article

Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

Insert

ArchT

Tags

EGFP

Expression

Mammalian

Mutation

ArchT is a more sensitive version of Arch (archae…

Promoter

Tet responsive Ptight promoter

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only
pT2/CMV-eGFP.PB/PTK

Plasmid

#170540

Purpose

Expresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restored

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

Delta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-mqgDUX4

Plasmid

#21176

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120393

Purpose

pAAV plasmid expressing Diras2 shRNA1 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter

Depositor

Florian Freudenberg

Article

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Insert

Diras2 shRNA (Diras2 Mouse)

Use

AAV and RNAi

Tags

mCherry.3XFLAG

Promoter

U6

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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mKikGR-Tubulin-6

Plasmid

#57330

Purpose

Localization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Tubulin (TUBA1B Human)

Tags

mKikGR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pBT259_(pRosa26-G-tTA2)

Plasmid

#36878

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
diphteria toxin A

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCA-G-intron(Neo)-T(FLLFLNL)

Plasmid

#40020

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tdT-3Myc
beta-globin intron
Neo

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pBT250_(pCA-G-intron(Neo)-tTA2)

Plasmid

#36876

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

Plasmid

#172581

Purpose

For transient expression of C-terminal BirA-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Tags

BirA(R118G)-HA

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4D RNAiR

Plasmid

#51600

Purpose

expresses RNAi-resistant mouse Sema4D with N-terminal myc tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D (Sema4d Mouse)

Tags

myc

Expression

Mammalian

Mutation

silent mutations between amino acids (approx 275-…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-P129D

Plasmid

#33084

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

P129D

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only