We narrowed to 1,794 results for: PORA

1. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

   Plasmid

   #89873

   Purpose

   expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   bArrestin2-TevC-P2A-TdTomato
   

   Use

   AAV

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   R173H in TdTomato (Please see depositor comments …

   Promoter

   Human synapsin

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRXIB-mRuby3-LC3

   Plasmid

   #221738

   Purpose

   Stable expression of mRuby3-LC3 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

   Plasmid

   #90008

   Purpose

   Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   NBirA*-linker-FKBP
   FRB-linker-CBirA*
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir

   Plasmid

   #90004

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   FKBP-linker-Myc-NBirA*
   CBirA*-linker-FRB
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EFS-DIO-GRAB_OT1.0

   Plasmid

   #185387

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin sensor GRAB_OT1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMIR-DsRed-IRES-His-Halo-Tev-Keap1

   Plasmid

   #58240

   Purpose

   expresses halo tagged human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

   Insert

   Kelch like ECH associated protein 1 (KEAP1 Human)
   

   Use

   Tags

   His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pLV-EF1A-LivePAR-Hygro

   Plasmid

   #176063

   Purpose

   EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   LivePAR
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FKBP-epsin(144-575)-GFP

   Plasmid

   #100733

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   epn1 (Epn1 Mouse)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   clathrin binding middle part aa 144-575

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR3.1-mGM-CSF

   Plasmid

   #74465

   Purpose

   expression of mGM-CSF in mammalian cells

   Depositor

   Loree Heller

   Article

   Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

   Insert

   granulocyte-monocyte colony-stimulating factor (Csf2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Sox2-CreERT2

    Plasmid

    #135618

    Purpose

    Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    P2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    P2A-CreERT2

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Mfn2-YFP

    Plasmid

    #28010

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

    Insert

    mitofusin 2 (MFN2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    silent S508S

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. CD8-mCherry-FRB

    Plasmid

    #100738

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    FRB and mCherry

    Expression

    Mammalian

    Mutation

    Truncated

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET22b-T5-sfGFP*- MmH-MH4R

    Plasmid

    #196409

    Purpose

    Express sfGFP with TAG and enzymes for biosynthesizing DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    sfGFP with TAG at 151 position
    Tyrosine Hydroxylase (Th Mouse)
    dihydropterine reductase from human (QDPR Human)
    pterin-4a-carbinolamine dehydratase from human
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

    Plasmid

    #90009

    Purpose

    Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    FKBP-linker-Myc-NBirA*
    FRB-linker-CBirA*
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-IP/Venus-rLC3

    Plasmid

    #58740

    Purpose

    Expresses Venus-rLC3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pLV-Hygro-EF1A-OB-Linker-eGFP

    Plasmid

    #176068

    Purpose

    EGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    OB
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-Hygro-EF1A-FHA-Linker-eGFP

    Plasmid

    #176065

    Purpose

    EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    FHA
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla

    Plasmid

    #174620

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a cone arrestin promoter

    Depositor

    Volker Busskamp

    Article

    Gasparini et al J Clin Invest. 2022 Apr 28. pii: 154619.

    Insert

    enhanced green fluorescent protein
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

    Plasmid

    #90003

    Purpose

    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    NBirA*-linker-FKBP
    CBirA*-linker-FRB
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSC-mCh-P2A-ST-EGFP-CAAX

    Plasmid

    #207636

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amber stop codon at SpyCatcher’s critical lysine

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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