We narrowed to 2,449 results for: PORA
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Plasmid#207637PurposeA plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.DepositorInsertEGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
ExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Aga2p-LOV-Turbo1-myc_pCTCON2
Plasmid#199673Purposeexpresses pre-evolved version of LOV-Turbo on the yeast surfaceDepositorInsertLOV-Turbo1
TagsAga2p and MycExpressionYeastAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
iEscSnFR
Plasmid#182807PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR
Tags6xHis, HA and MycExpressionBacterialPromoterT7Available SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
iFluoxSnFR
Plasmid#182808PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR
Tags6xHis, HA and MycExpressionBacterialPromoterT7Available SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTV-Flnc-5prime-1000-24MS96T-3F_NeoR
Plasmid#174881PurposeTargeting vector for generating Flnc STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Flnc
UseMouse TargetingAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTV-Usp5-5prime-1000-24MS96T-3F_NeoR
Plasmid#174885PurposeTargeting vector for generating Usp5 STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Usp5
UseMouse TargetingAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR
Plasmid#174886PurposeTargeting vector for generating Wnk1 STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Wnk1
UseMouse TargetingAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-D27S/Y100F
Plasmid#110827PurposeBacterial expression of E.coli DHFR D27S/Y100F mutantDepositorInsertecDHFR
ExpressionBacterialMutationD27 mutated to S27, Y100 mutated to F100PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)
Plasmid#107739Purposemutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein(C1A)
ExpressionWormMutationchange the first cysteine of gp41-1 N-intein to a…PromoterC. elegans myo-2 promoterAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3
Plasmid#171150PurposeCation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2f-P2A-H2B-mRuby3
UseAAVExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NDP52
Plasmid#135296Purposestably express GFP-tagged NDP52 for CID induced mitophagyDepositorAvailable SinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mfn2-YFP
Plasmid#28010DepositorAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT3.0
Plasmid#208717PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in neuronsDepositorHas ServiceAAV1, AAV2, and AAV8InsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVPromoterhSynAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin- soCoChR-GFP
Plasmid#107708PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoterDepositorHas ServiceAAV9InsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianPromoterhSynapsinAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh
Plasmid#207634PurposeA plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.DepositorInsertphotocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
ExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
ppk301-T2A-QF2 HDR plasmid
Plasmid#130667PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 geneDepositorInsertppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
UseDonor templateAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa
Plasmid#205766PurposeExpression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR Clover, and P38 KTR Dronpa in mamalian cellsDepositorInsertJNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa
UseAAVExpressionMammalianMutationwtAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-hsp70I:QFGal4-SV40pA
Plasmid#155120PurposeTol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInserthsp70l:QFGal4 (hsp70l Neurospora crassa, Budding Yeast, Zebrafish)
UseZebrafish expressionPromoterhsp70lAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-CBir-FRB_Myc-NBir-FKBP
Plasmid#90003PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
CBirA*-linker-FRB
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3
Plasmid#170177PurposeCation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2s-ST-P2A-NLS-mRuby3
UseAAVExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only