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We narrowed to 63,651 results for: cloning

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Showing: 1141 - 1160 of 63651 results

  1. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107942-plasmid-map-sequence-id-259760

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  2. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid
    #89355
    Purpose
    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs
    Depositor
    Feng Zhang
    Publication
    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cpf1, RR variant
    Use
    CRISPR
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    G532R/K595R
    Promoter
    CMV
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89355-plasmid-map-sequence-id-178226

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  3. pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

    Plasmid
    #79620
    Purpose
    Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells
    Depositor
    Akihiko Kondo
    Publication
    Nishida et al Science. 2016 Aug 4. pii: aaf8729.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Tags
    PmCDA1 and UGI
    Expression
    Mammalian
    Mutation
    D10A for nickase Cas9
    Promoter
    pCMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79620-plasmid-map-sequence-id-154120

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  13. pDEST51-LRRK2-G2019S

    Plasmid
    #29401
    Depositor
    Mark Cookson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LRRK2 (LRRK2 Human)
    Use
    Gateway Cloning
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Gly2019Ser
    Available since
    April 1, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29401-plasmid-map-sequence-id-256667

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Showing: 1141 - 1160 of 63651 results