We narrowed to 62,126 results for: cloning
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Plasmid#183097PurposeFor plant prime editing in rice plantsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRExpressionPlantMutationD10A in SpCas9Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-2xFYVE
Plasmid#140050PurposeExpresses mCherry-tagged HRS 2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain (Hgs Mouse)
TagsmCherryExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterCMVAvailable SinceMay 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-CBE N-terminal
Plasmid#137175PurposeAAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE N-terminal; U6-protospacer
UseAAVMutationCas9 D10APromoterCbhAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-15F11-HA-Halo
Plasmid#129592PurposeThe encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
ExpressionMammalianPromoterCMVAvailable SinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-CMV-3xFLAG-gateway-EGFP
Plasmid#122845PurposeN-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway CloningTags3xFLAG and EGFPExpressionMammalianPromoterCMVAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETA
Plasmid#53272PurposeContains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertcrtE, crtY, crtI, crtB
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-H2B-GCaMP6s
Plasmid#59530PurposeExpressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTransposable elementAvailable SinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMYC-GFP
Plasmid#42142DepositorAvailable SinceOct. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST/hCx43-EGFP-N1
Plasmid#40907DepositorInsertConnexin 43 (GJA1 Human)
TagsEGFPExpressionMammalianMutationNo stop codon to create c-terminal fusionPromoterCMVAvailable SinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRExpressionBacterialAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH029
Plasmid#171060PurposePlasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in betweenDepositorInsertGag-spyCas9
UseCRISPR and LentiviralTags2x SV40 NLS and 3x FLAG tagExpressionMammalianPromoterCAGAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-IRES-puro
Plasmid#128652Purposemammalian expression of EGFP with puromycin selectionDepositorInsertEGFP
UseLentiviralTagsN/AExpressionMammalianMutationWild typePromoterCMVAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-hNIL
Plasmid#105841PurposeDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuronDepositorInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B
Plasmid#103002Purposenon-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAMD1-2
Plasmid#61564PurposeStandardized mini-Tn5 delivery plasmid, kanamycin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorAvailable SinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9
Plasmid#46920PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoterDepositorInsertdCas9
UseCRISPRExpressionYeastPromoterTDH3Available SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MET
Plasmid#23889DepositorInsertMET (MET Human)
UseGateway CloningAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ALK
Plasmid#23917DepositorInsertALK (ALK Human)
UseGateway CloningAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Rab8A[T22N]
Plasmid#24899DepositorInserthRab8A[T22N]
TagsGFPExpressionMammalianMutationChanged Threonine 22 to AsparagineAvailable SinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_R280K
Plasmid#22933DepositorInserttumor protein 53 (TP53 Human)
UseLentiviralTagsv5ExpressionMammalianMutationR280K mutantAvailable SinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only