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We narrowed to 16,181 results for: nes

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Showing: 1141 - 1160 of 16181 results

  1. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

    Plasmid
    #119723
    Purpose
    AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons
    Depositor
    Thomas Oertner
    Publication
    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:
    Inserts
    CaMPARI2
    CaMPARI2
    Use
    AAV
    Tags
    NES-his
    Expression
    Mammalian
    Mutation
    F391W, L398V
    Promoter
    hSyn1
    Available since
    Jan. 31, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    119723-plasmid-map-sequence-id-232115

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  5. pAAV gfaABC1D:NES-jRGECO1a

    Plasmid
    #239682
    Purpose
    Red calcium indicator jRGECO1a with a nuclear export signal expressed under an astrocyte-specific promoter.
    Depositor
    Marianne Fyhn
    Insert
    jRGECO1a
    Use
    AAV
    Tags
    NES
    Expression
    Mammalian
    Promoter
    gfaABC1D
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    239682-plasmid-map-sequence-id-478760

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  6. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid
    #181974
    Purpose
    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
    Use
    Lentiviral
    Mutation
    NGFR truncation: aa 11-276
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    181974-plasmid-map-sequence-id-355578

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  7. lentiGuideFE-Puro

    Plasmid
    #170069
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter
    Depositor
    Neville Sanjana
    Publication
    Morris et al Science. 2023 May 19;380(6646):eadh7699.
    Insert
    Cas9 guide RNA scaffold with the F+E scaffold modification
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    170069-plasmid-map-sequence-id-333408

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  8. lentiCRISPR - EGFP sgRNA 5

    Plasmid
    #51764
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 5
    Use
    CRISPR and Lentiviral
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    51764-plasmid-map-sequence-id-79386

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  11. RANLS-LEHD-BNES

    Plasmid
    #60973
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A and ddRFP B
    Tags
    Caspase9 substrate sequence (LEHD) is after NLS s…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. lentiCRISPR - EGFP sgRNA 6

    Plasmid
    #51765
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    51765-plasmid-map-sequence-id-79389

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  15. pENTR_L1-miniTurbo-NES-YFP-STOP-L2

    Plasmid
    #127359
    Purpose
    gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, NES, V5, and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127359-plasmid-map-sequence-id-246306

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  16. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174902-plasmid-map-sequence-id-344602

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  17. RANLS-IETD-BNES

    Plasmid
    #60972
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A and ddRFP B
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1141 - 1160 of 16181 results