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UL79-3xFLAG pCDNA4

Plasmid

#120374

Purpose

Expresses HCMV UL79 with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL79 (UL79 Human Cytomegalovirus (HHV-5))

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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FUp95bGW (B6)

Plasmid

#74015

Purpose

lentiviral expression of shRNA resistant Psd95-beta-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 beta isoform

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant Psd95

Promoter

hUbC

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
hSgk1_v3 Flag

Plasmid

#86039

Purpose

full length human Sgk1_i3 with Flag tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2012 Dec 1;3

Insert

Alternate transcript v3 of human Sgk1 (SGK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

v3; S313G

Promoter

CMV

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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vector-dendra2 PCDNA

Plasmid

#121134

Purpose

mammalian cell expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDD375

Plasmid

#91825

Purpose

Codon-optimized mKate2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mKate2-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-EGFP-HsNanos2_6-22_X

Plasmid

#147929

Purpose

Mammalian Expression of HsNanos2_6-22

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2_6-22 (NANOS2 Human)

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Ptac_P

Plasmid

#127004

Purpose

Basic Part P- Promoter (non-constitutive); Ptac; IPTG/lactose induced; controlled by LacI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Ptac_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6p-PBX1

Plasmid

#78145

Purpose

bacterial expression of GST-PBX1

Depositor

Francesco Blasi

Article

Mathiasen et al PLoS One. 2015 Apr 9;10(4):e0125789. doi

Insert

PBX1 (PBX1 Human)

Tags

GST

Expression

Bacterial

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
YIplac211-STV1-iGFP

Plasmid

#177111

Purpose

Expresses Stv1-iGFP in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

STV1 (STV1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-28

Plasmid

#120007

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-Suv39H2_3

Plasmid

#36346

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H2 (SUV39H2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pAP635-2

Plasmid

#99485

Purpose

Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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RIT2

Plasmid

#55663

Purpose

expression of mouse RIT2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RIT2 (Rit2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
AIRAP 11: PSMD14.pEYFP.V1

Plasmid

#21801

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD14 (Psmd14 Mouse)

Tags

YFP

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODAL-matMYC

Plasmid

#115639

Purpose

For targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Keratin 18 S33A

Plasmid

#18061

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Mutation

S33A

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pMX1433

Plasmid

#127857

Purpose

Expresses EGFP-deltaN-term-MCAK in mammalian cells

Depositor

Linda Wordeman

Article

Domnitz et al J Cell Biol. 2012 Apr 16;197(2):231-7. d

Insert

deltaN-term-MCAK

Tags

EGFP

Expression

Mammalian

Mutation

deletion of first 19 codons of MCAK

Promoter

CMV

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 2

Plasmid

#8471

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

261DLF263-AAA (D261A, L262A, F263A)

Available Since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
MLM3367

Plasmid

#43884

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NI (binds A)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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RCAS (A) cTbx3 del2 (CT#645)

Plasmid

#13921

Depositor

Cliff Tabin

Article

Logan et al Development. 1998 Aug . 125(15):2825-35.

Insert

Tbx3 del (TBX3 Chicken)

Use

Retroviral; Avian expression

Mutation

Truncated Tbx3. Putative dominant negative.

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only