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We narrowed to 6,185 results for: cas9 expression plasmid

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Showing: 5811 - 5820 of 6185 results

  5. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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  6. pGG184

    Plasmid
    #165604
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    'CAGCG' PAM replacing the 'CGGCG&#…
    Promoter
    lac
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165604-plasmid-map-sequence-id-334628

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  7. psPAX2-D64V-NC-MS2

    Plasmid
    #122944
    Purpose
    MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles
    Depositor
    Baisong Lu
    Article
    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531
    Insert
    NC-MCP (MS2 coat protein) fusion protein in Gag
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    D64V
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122944-plasmid-map-sequence-id-239322

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  8. psPAX2-D64V-NC-PP7

    Plasmid
    #122945
    Purpose
    PP7 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an PP7 aptamer into lentivirus-like particles
    Depositor
    Baisong Lu
    Article
    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531
    Insert
    NC-PCP (PP7 coat protein) fusion protein in Gag
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    D64V
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122945-plasmid-map-sequence-id-239315

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  9. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

    Plasmid
    #239102
    Purpose
    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.
    Depositor
    Karl Wahlin
    Article
    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472
    Insert
    POU4F2 (POU4F2 Human)
    Use
    Donor plasmid for integration into the human geno…
    Tags
    p2A-tdTomato-p2A-Thy1.2
    Expression
    Mammalian
    Promoter
    Endogenous POU4F2
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239102-plasmid-map-sequence-id-474475

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Showing: 5811 - 5820 of 6185 results