We narrowed to 10,453 results for: yeast

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pCRISPEY-Z3-Nat

Plasmid

#232100

Purpose

Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCTcon2-5INS_16

Plasmid

#102292

Purpose

Yeast Surface Display of 5Ins_16 scFV

Depositor

Sarel Fleishman

Article

Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

Insert

Insulin binding scFV

Tags

3x(G4S) Linker, G3S Linker, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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12URA-B-ScTop2∆CTD

Plasmid

#214924

Purpose

Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

Depositor

James Berger

Article

Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

Insert

topoisomerase II

Tags

6x His-TEV

Expression

Yeast

Mutation

Deleted amino acids 1178-1428

Promoter

Gal 1/10

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-OSBP PH domain

Plasmid

#58840

Purpose

Expresses GFP-Tagged OSBP PH domain in yeast

Depositor

Tim Levine, Sean Munro

Article

Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

Insert

Oxysterol-binding Protein PH domain (OSBP Human)

Tags

GFP

Expression

Yeast

Promoter

PHO5

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pRS426-GFP-Osh2-PH

Plasmid

#71373

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pRS406 ATG9 stopGFP

Plasmid

#114669

Purpose

Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Mutation

Changed the three linker base pairs after the las…

Promoter

ATG9

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCTcon2-5INS_EV3

Plasmid

#102293

Purpose

Yeast Surface Display of 5Ins_16_EV3

Depositor

Sarel Fleishman

Article

Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

Insert

Evolved Insulin binding scFV

Tags

3x(G4S) Linker, G3S Linker, HA, and Myc

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pG23A

Plasmid

#102884

Purpose

Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHO_pTEFmut7_CggR_R250A_ble

Plasmid

#124585

Purpose

Plasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoter

Depositor

Matthias Heinemann

Article

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

cggR_R250A (cggR Synthetic)

Use

Synthetic Biology

Mutation

changed arginine 250 to alanine

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Osh1-PH

Plasmid

#71372

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh1-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa267-388

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only