We narrowed to 15,621 results for: nol

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pSELECT-HA-mFOXO1

Plasmid

#83308

Purpose

Expresses HA-tagged mouse FoxO1 in mammalian cells, PuroR selection marker

Depositor

Steven Abcouwer

Article

Kong et al Exp Eye Res. 2016 Aug 7;151:82-95. doi:

Insert

FoxO1 (Foxo1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

hEF1/HTLV prom

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-hAID-BE3

Plasmid

#113417

Purpose

Expresses hAID-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hAID-BE3 (AICDA Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF655

Plasmid

#88578

Purpose

Donor Vector containing ZNF655 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF655 (ZNF655 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Grb2-SH2

Plasmid

#46440

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb2 (GRB2 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 58-159

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(NC)-SH2

Plasmid

#46471

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(NC) (PLCG1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 542-759

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pJFA334E9

Plasmid

#49237

Purpose

TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

TET1 tet methylcytosine dioxygenase 1 (TET1 Human, Synthetic)

Use

TALEN; Tale-tet1 expression vector

Promoter

EF1a

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-P2A-GFP-PGK-Puro

Plasmid

#110861

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF736

Plasmid

#101602

Purpose

Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF736 (ZNF736 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAG106 Lyn11-FAST

Plasmid

#130721

Purpose

Expresses FAST (also called YFAST) fused to Lyn11 sequence in mammalian cells

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

lyn11-FAST

Tags

myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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RPS6KA3 gRNA (BRDN0001144943)

Plasmid

#75942

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA3 (RPS6KA3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-MIOS(1-875) in pRK5

Plasmid

#184561

Purpose

CMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.

Depositor

Kacper Rogala

Article

Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

Insert

MIOS (MIOS Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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SS80 pEGFPN1 anti-SAPAP2 nanobody

Plasmid

#135228

Purpose

Mammalian expression of anti-SAPAP (Rat) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS80 (Dlgap2 L. glama (llama))

Tags

6xHis-HA-EGFP

Expression

Mammalian

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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RPS6KA3 gRNA (BRDN0001148452)

Plasmid

#75943

Purpose

3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RPS6KA3 (RPS6KA3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (GAPDH)

Plasmid

#170120

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (GAPDH Human)

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-MMACHC [N230/21R]

Plasmid

#114555

Purpose

Mammalian Expression Plasmid of anti-MMACHC (Human). Derived from hybridoma N230/21.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MMACHC (Homo sapiens) recombinant mouse monoclonal antibody (MMACHC Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAG156 Mito-FAST

Plasmid

#130723

Purpose

Expresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Mito-FAST

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Olig1 [N149/25.1R]

Plasmid

#114540

Purpose

Mammalian Expression Plasmid of anti-Olig1 (Mouse). Derived from hybridoma N149/25.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Olig1 (Mus musculus) recombinant mouse monoclonal antibody (Olig1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-5 [N195A/16R]

Plasmid

#114481

Purpose

Mammalian Expression Plasmid of anti-QKI-5 (Human). Derived from hybridoma N195A/16.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-QKI-5 (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)

Plasmid

#139447

Purpose

Lentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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SPACE-VRQR (pRZ5137)

Plasmid

#140245

Purpose

CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

SPACE_VRQR

Expression

Mammalian

Mutation

V82G in TadA*, VRQR mutations in SpCas9, D10A in …

Promoter

CMV

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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